Department of Genetics, University of Pretoria, Pretoria 0002, South Africa.
Mycopathologia. 2010 May;169(5):413-23. doi: 10.1007/s11046-009-9267-8. Epub 2010 Jan 7.
Polymorphic sequence-characterised marker assays from a recent diversity study on the Ascomycete fungus Ophiostoma quercus reported that some isolates from Africa were genetically distinct from O. quercus. In the present study, these African isolates were compared with authentic O. quercus isolates by evaluating morphological characters, growth in culture, mating compatibility and DNA sequence data. The isolates from Africa were morphologically similar to O. quercus, presenting Pesotum and Sporothrix synanamorphs in culture. Phylogenetic analyses of the ribosomal internal transcribed spacer regions 1 and 2, beta-tubulin and translation elongation factor 1-alpha gene regions confirmed that the African group represents a distinct species within the hardwood lineage of the O. piceae complex, closely related to O. ulmi and O. himal-ulmi. Mating studies between O. quercus and the African isolates showed that isolates mated predominantly with those of their own group, although there were rare cases of fertile crosses between the groups. Isolates residing in the African lineage are described here as a new species, O. tsotsi sp. nov.
近期对壳囊孢目真菌（Ophiostoma）的多样性研究中，采用多态性序列特征标记分析，报道称一些来自非洲的分离物在遗传上与栓皮栎壳囊孢（O. quercus）不同。本研究通过评估形态特征、培养中的生长情况、交配亲和性和 DNA 序列数据，将这些非洲分离物与真正的 O. quercus 分离物进行了比较。来自非洲的分离物在形态上与 O. quercus 相似，在培养中呈现 Pesotum 和 Sporothrix 同宗配合现象。核糖体内部转录间隔区 1 和 2、β-微管蛋白和翻译延伸因子 1-α 基因区域的系统发育分析证实，非洲群代表硬木谱系 O. piceae 复合体中的一个独特种，与 O. ulmi 和 O. himal-ulmi 密切相关。O. quercus 和非洲分离物之间的交配研究表明，分离物主要与自身群体的分离物交配，但也有两个群体之间偶尔出现可育杂交的情况。居住在非洲谱系中的分离物被描述为一个新种，即 O. tsotsi sp. nov.。
