Department of Microbiology and Plant Pathology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa;
Persoonia. 2010 Dec;25:94-108. doi: 10.3767/003158510X551097. Epub 2010 Dec 14.
Leptographium spp. are anamorphs of Grosmannia residing in the order Ophiostomatales. These fungi are typically associated with bark-beetles and are common causal agents of sapstain in lumber and some are important tree pathogens. In this study, Leptographium spp. associated with bark beetles collected during a survey in Jilin and Yunnan provinces of China, were identified. Identifications were achieved using comparisons of morphological characters and DNA sequence data for the ITS2-partial LSU rDNA region, as well as the β-tubulin and EF-1α gene regions. Eight unknown species of Leptographium are recognised and described from conifer and hardwood hosts, associated with beetles including Ips subelongatus, Tomicus yunnanensis, Hylurgops minor, Polygraphus verrucifrons and a Pissodes sp. Six of the new species are morphologically and phylogenetically related to species known to occur in Asia such as G. yunnanense, L. bhutanense, L. bistatum and L. sinoprocerum. The remaining two taxa are related to those in a group containing G. americana and L. abietinum, found in North America. This study also provides the first report of L. pineti on Pinus kesiya in China.
Leptographium spp.是 Grosmannia 的无性型，属于 Ophiostomatales 目。这些真菌通常与树皮甲虫有关，是木材中 sapstain 的常见病原体，有些还是重要的树木病原体。在这项研究中，对在中国吉林和云南两省进行调查时收集到的与树皮甲虫有关的 Leptographium spp.进行了鉴定。鉴定是通过比较 ITS2-部分 LSU rDNA 区域、β-微管蛋白和 EF-1α 基因区域的形态特征和 DNA 序列数据来实现的。从针叶树和阔叶树宿主中鉴定并描述了 8 个未知的 Leptographium 种，与包括 Ips subelongatus、Tomicus yunnanensis、Hylurgops minor、Polygraphus verrucifrons 和 Pissodes sp. 在内的甲虫有关。其中 6 个新种在形态和系统发育上与亚洲已知的种有关，如 G. yunnanense、L. bhutanense、L. bistatum 和 L. sinoprocerum。其余两个分类群与包含 G. americana 和 L. abietinum 的北美组中的种有关。本研究还首次报道了在中国的 Pinus kesiya 上的 L. pineti。
