Winograd Nicholas, Garrison Barbara J
Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
Annu Rev Phys Chem. 2010;61:305-22. doi: 10.1146/annurev.physchem.040808.090249.
This article reviews the new physics and new applications of secondary ion mass spectrometry using cluster ion probes. These probes, particularly C(60), exhibit enhanced molecular desorption with improved sensitivity owing to the unique nature of the energy-deposition process. In addition, these projectiles are capable of eroding molecular solids while retaining the molecular specificity of mass spectrometry. When the beams are microfocused to a spot on the sample, bioimaging experiments in two and three dimensions are feasible. We describe emerging theoretical models that allow the energy-deposition process to be understood on an atomic and molecular basis. Moreover, experiments on model systems are described that allow protocols for imaging on biological materials to be implemented. Finally, we present recent applications of imaging to biological tissue and single cells to illustrate the future directions of this methodology.
本文综述了使用团簇离子探针的二次离子质谱的新物理和新应用。这些探针，特别是C(60)，由于能量沉积过程的独特性质，表现出增强的分子解吸并具有更高的灵敏度。此外，这些射弹能够侵蚀分子固体，同时保持质谱的分子特异性。当束流微聚焦到样品上的一个点时，二维和三维生物成像实验是可行的。我们描述了新兴的理论模型，这些模型能让我们在原子和分子层面理解能量沉积过程。此外，还描述了在模型系统上进行的实验，这些实验使在生物材料上成像的方案得以实施。最后，我们展示了成像技术在生物组织和单细胞上的最新应用，以说明该方法的未来发展方向。
