Department of Chemistry, Pennsylvania State University, University Park, Pennsylvania 16802, USA; email:
Annu Rev Anal Chem (Palo Alto Calif). 2018 Jun 12;11(1):29-48. doi: 10.1146/annurev-anchem-061516-045249. Epub 2018 Feb 28.
Gas cluster ion beams (GCIBs) provide new opportunities for bioimaging and molecular depth profiling with secondary ion mass spectrometry (SIMS). These beams, consisting of clusters containing thousands of particles, initiate desorption of target molecules with high yield and minimal fragmentation. This review emphasizes the unique opportunities for implementing these sources, especially for bioimaging applications. Theoretical aspects of the cluster ion/solid interaction are developed to maximize conditions for successful mass spectrometry. In addition, the history of how GCIBs have become practical laboratory tools is reviewed. Special emphasis is placed on the versatility of these sources, as size, kinetic energy, and chemical composition can be varied easily to maximize lateral resolution, hopefully to less than 1 micron, and to maximize ionization efficiency. Recent examples of bioimaging applications are also presented.
气体团簇离子束（GCIBs）为利用二次离子质谱（SIMS）进行生物成像和分子深度剖析提供了新的机会。这些束流由包含数千个粒子的团簇组成，能够以高产率和最小的碎片化引发靶分子的解吸。本综述强调了实施这些源的独特机会，特别是对于生物成像应用。开发了团簇离子/固体相互作用的理论方面，以最大限度地提高成功进行质谱分析的条件。此外，还回顾了 GCIB 如何成为实用实验室工具的历史。特别强调了这些源的多功能性，因为可以轻松改变尺寸、动能和化学成分，以最大限度地提高横向分辨率，希望达到小于 1 微米，并最大限度地提高离子化效率。还介绍了最近的生物成像应用实例。
