Fontaine Benjamin M, Nelson Kate, Lyles James T, Jariwala Parth B, García-Rodriguez Jennifer M, Quave Cassandra L, Weinert Emily E
Department of Chemistry, Emory University Atlanta, GA, USA.
Department of Dermatology, Emory University School of Medicine Atlanta, GA, USA.
Front Microbiol. 2017 Mar 23;8:496. doi: 10.3389/fmicb.2017.00496. eCollection 2017.
is a leading cause of hospital-acquired infections. It is listed among the top "serious threats" to human health in the USA, due in large part to rising rates of resistance. Many infections are recalcitrant to antibiotic therapy due to their ability to form a biofilm, which acts not only as a physical barrier to antibiotics and the immune system, but results in differences in metabolism that further restricts antibiotic efficacy. Development of a modular strategy to synthesize a library of phenolic glycosides allowed for bioactivity testing and identification of anti-biofilm compounds within an extract of the elmleaf blackberry (). Two ellagic acid (EA) derivatives, EA xyloside and EA rhamnoside, have been identified as components of the extract. In addition, EA rhamnoside has been identified as an inhibitor of biofilm formation, with activity comparable to the complex extract 220D-F2 (composed of a mixture of EA glycosides), and confirmed by confocal laser scanning microscopy analyses.
是医院获得性感染的主要原因。它被列为美国对人类健康的“严重威胁”之一，这在很大程度上归因于耐药率的上升。许多感染对抗生素治疗具有顽固性，因为它们能够形成生物膜，生物膜不仅作为抗生素和免疫系统的物理屏障，而且还导致新陈代谢的差异，进一步限制了抗生素的疗效。开发一种模块化策略来合成酚苷文库，以便对榆叶黑莓提取物中的抗生物膜化合物进行生物活性测试和鉴定。两种鞣花酸（EA）衍生物，EA木糖苷和EA鼠李糖苷，已被鉴定为提取物的成分。此外，EA鼠李糖苷已被鉴定为生物膜形成的抑制剂，其活性与复杂提取物220D-F2（由EA糖苷混合物组成）相当，并通过共聚焦激光扫描显微镜分析得到证实。
