Gursale Atish, Dighe Vidya, Parekh Guarang
Ramnarain Ruia College, Department of Chemistry, Mumbai, India.
J Chromatogr Sci. 2010 Jan;48(1):59-62. doi: 10.1093/chromsci/48.1.59.
A simple, sensitive, and precise reversed-phase high performance liquid chromatographic (HPLC) method has been developed, validated and used for simultaneous quantitative determination of cinnamaldehyde and methyl eugenol from the methanolic extract of dried bark powder of Cinnamomum zeylanicum Blume (family Lauraceae). The ultrasonic extraction method was used for the extraction of these compounds. The reversed-phase HPLC analysis was carried out using a Intersil ODS-3V-C(18) (150 mm x 4.6 mm, 5 microm) column and a mobile phase comprising of methanol-acetonitrile-water in the volume ratio of 35:20:45, delivered at a flow rate of 1.0 cm(3)/min. The detection and quantitation of both the compounds was carried out at 221 nm.
已开发、验证并使用一种简单、灵敏且精确的反相高效液相色谱（HPLC）方法，用于同时定量测定锡兰肉桂（樟科）干燥树皮粉末甲醇提取物中的肉桂醛和甲基丁香酚。采用超声提取法提取这些化合物。反相HPLC分析使用Intersil ODS - 3V - C(18)（150 mm×4.6 mm，5微米）色谱柱，流动相由体积比为35:20:45的甲醇 - 乙腈 - 水组成，流速为1.0 cm³/min。两种化合物的检测和定量均在221 nm波长下进行。
