Chatterjee Kaushik, Young Marian F, Simon Carl G
Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Comb Chem High Throughput Screen. 2011 May;14(4):227-36. doi: 10.2174/138620711795222455.
Optimizing cell-material interactions is critical for maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We have developed a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we provide detailed instructions on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.
优化细胞与材料的相互作用对于在组织工程中实现最大程度的再生至关重要。组合和高通量（CHT）方法可用于系统地筛选组织支架，以确定最佳的生物材料特性。先前组织工程中的CHT平台涉及二维（2D）细胞培养形式，即细胞在材料表面上培养。然而，这些平台不足以预测三维（3D）组织支架中的细胞反应。我们开发了一种简单的CHT平台，以三维培养形式筛选细胞与材料的相互作用，该平台可用于筛选水凝胶支架。在此，我们提供了一种制备可光聚合水凝胶弹性模量梯度的方法的详细说明。
