制备用于三维细胞培养的梯度水凝胶支架。
Fabricating gradient hydrogel scaffolds for 3D cell culture.
作者信息
Chatterjee Kaushik, Young Marian F, Simon Carl G
机构信息
Polymers Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
出版信息
Comb Chem High Throughput Screen. 2011 May;14(4):227-36. doi: 10.2174/138620711795222455.
Abstract
Optimizing cell-material interactions is critical for maximizing regeneration in tissue engineering. Combinatorial and high-throughput (CHT) methods can be used to systematically screen tissue scaffolds to identify optimal biomaterial properties. Previous CHT platforms in tissue engineering have involved a two-dimensional (2D) cell culture format where cells were cultured on material surfaces. However, these platforms are inadequate to predict cellular response in a three-dimensional (3D) tissue scaffold. We have developed a simple CHT platform to screen cell-material interactions in 3D culture format that can be applied to screen hydrogel scaffolds. Herein we provide detailed instructions on a method to prepare gradients in elastic modulus of photopolymerizable hydrogels.
摘要
优化细胞与材料的相互作用对于在组织工程中实现最大程度的再生至关重要。组合和高通量（CHT）方法可用于系统地筛选组织支架，以确定最佳的生物材料特性。先前组织工程中的CHT平台涉及二维（2D）细胞培养形式，即细胞在材料表面上培养。然而，这些平台不足以预测三维（3D）组织支架中的细胞反应。我们开发了一种简单的CHT平台，以三维培养形式筛选细胞与材料的相互作用，该平台可用于筛选水凝胶支架。在此，我们提供了一种制备可光聚合水凝胶弹性模量梯度的方法的详细说明。
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本文引用的文献
1
Soft biological materials and their impact on cell function.
Soft Matter. 2007 Feb 14;3(3):299-306. doi: 10.1039/b610522j.
2
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3
Cell interactions with biomaterials gradients and arrays.
Comb Chem High Throughput Screen. 2009 Jul;12(6):544-53. doi: 10.2174/138620709788681961.
4
Osteoblast response to dimethacrylate composites varying in composition, conversion and roughness using a combinatorial approach.
Biomaterials. 2009 Sep;30(27):4480-7. doi: 10.1016/j.biomaterials.2009.05.019. Epub 2009 Jun 10.
5
Physical approaches to biomaterial design.
Nat Mater. 2009 Jan;8(1):15-23. doi: 10.1038/nmat2344.
6
Substrate modulus directs neural stem cell behavior.
Biophys J. 2008 Nov 1;95(9):4426-38. doi: 10.1529/biophysj.108.132217. Epub 2008 Jul 25.
7
Three-dimensional extracellular matrix-directed cardioprogenitor differentiation: systematic modulation of a synthetic cell-responsive PEG-hydrogel.
Biomaterials. 2008 Jun;29(18):2757-66. doi: 10.1016/j.biomaterials.2008.03.016. Epub 2008 Apr 7.
8
The effects of matrix stiffness and RhoA on the phenotypic plasticity of smooth muscle cells in a 3-D biosynthetic hydrogel system.
Biomaterials. 2008 Jun;29(17):2597-607. doi: 10.1016/j.biomaterials.2008.02.005. Epub 2008 Mar 14.
9
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10
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Cell. 2007 Aug 24;130(4):601-10. doi: 10.1016/j.cell.2007.08.006.
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