Laboratory of Biochemical Engineering, School of Chemistry and Food, Federal University of Rio Grande, P.O. Box 474, 96201-900, Rio Grande, RS, Brazil.
Bioresour Technol. 2010 Apr;101(8):2872-6. doi: 10.1016/j.biortech.2009.11.059. Epub 2009 Dec 28.
Spirulina is a microalga which offers biological functions highly favorable for tissue engineering. Highly porous scaffolds can be produced by electrospinning containing biomass of Spirulina. The goal of this contribution was therefore to establish spinning conditions allowing to produce well defined nanofibers with diameters down to about 100 nm and to produce nanofibers with various concentration of the biomass for subsequent studies in tissue engineering applications. The experimental results reveal that the blend system PEO/biomass is behaved surprisingly well in electrospinning. Very thin bead-free nanofibers with diameters of about 110 nm can be produced for different biomass contents of up to 67 wt.% of the nanofibers and for PEO concentrations in the spinning solution well below 4 wt.%. These results suggest to us the use of the biomass containing nanofibers as extracellular matrices for stem cell culture and future treatment of spinal chord injury.
螺旋藻是一种微藻，具有高度有利于组织工程的生物功能。通过含有螺旋藻生物质的静电纺丝可以生产出具有高多孔性的支架。因此，本研究的目的是建立能够产生直径约为 100nm 的定义良好的纳米纤维的纺丝条件，并生产具有不同生物质浓度的纳米纤维，以便用于组织工程应用的后续研究。实验结果表明，PEO/生物质的共混体系在静电纺丝中表现出非常好的行为。对于不同的生物质含量高达 67wt%的纳米纤维，以及纺丝溶液中 PEO 浓度远低于 4wt%的情况下，都可以生产出非常细的无珠纳米纤维，直径约为 110nm。这些结果提示我们使用含有生物质的纳米纤维作为干细胞培养和未来脊髓损伤治疗的细胞外基质。
