拟南芥SDF2样蛋白的克隆、重组表达、结晶及初步X射线衍射分析
Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of SDF2-like protein from Arabidopsis thaliana.
作者信息
Radzimanowski Jens, Ravaud Stephanie, Schott Andrea, Strahl Sabine, Sinning Irmgard
机构信息
Heidelberg University Biochemistry Center (BZH), Im Neuenheimer Feld 328, D-69120 Heidelberg, Germany.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):12-4. doi: 10.1107/S1744309109042018. Epub 2009 Dec 25.
Abstract
The stromal-cell-derived factor 2-like protein of Arabidopsis thaliana (AtSDL) has been shown to be highly up-regulated in response to unfolded protein response (UPR) inducing reagents, suggesting that it plays a crucial role in the plant UPR pathway. AtSDL has been cloned, overexpressed, purified and crystallized using the vapour-diffusion method. Two crystal forms have been obtained under very similar conditions. The needle-shaped crystals did not diffract X-rays, while the other form diffracted to 1.95 A resolution using a synchrotron-radiation source and belonged to the hexagonal space group P6(1), with unit-cell parameters a = b = 96.1, c = 69.3 A.
摘要
拟南芥的基质细胞衍生因子2样蛋白（AtSDL）已被证明在响应未折叠蛋白反应（UPR）诱导试剂时高度上调，这表明它在植物UPR途径中起关键作用。AtSDL已被克隆、过表达、纯化并使用气相扩散法结晶。在非常相似的条件下获得了两种晶体形式。针状晶体不能衍射X射线，而另一种形式使用同步辐射源衍射到1.95 Å分辨率，属于六方空间群P6(1)，晶胞参数a = b = 96.1，c = 69.3 Å。
相似文献
1
Cloning, recombinant production, crystallization and preliminary X-ray diffraction analysis of SDF2-like protein from Arabidopsis thaliana.拟南芥SDF2样蛋白的克隆、重组表达、结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Jan 1;66(Pt 1):12-4. doi: 10.1107/S1744309109042018. Epub 2009 Dec 25.
2
Expression, purification, crystallization and preliminary X-ray diffraction analysis of Arabidopsis thaliana cyclophilin 38 (AtCyp38).拟南芥亲环蛋白38（AtCyp38）的表达、纯化、结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Dec 1;61(Pt 12):1087-9. doi: 10.1107/S1744309105037681. Epub 2005 Nov 24.
3
GPX3 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis.来自拟南芥的谷胱甘肽过氧化物酶3：克隆、表达、纯化、结晶及初步X射线分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Nov;69(Pt 11):1224-6. doi: 10.1107/S1744309113025566. Epub 2013 Oct 17.
4
Recombinant ACHT1 from Arabidopsis thaliana: crystallization and X-ray crystallographic analysis.来自拟南芥的重组ACHT1：结晶及X射线晶体学分析
Acta Crystallogr F Struct Biol Commun. 2017 Jul 1;73(Pt 7):382-385. doi: 10.1107/S2053230X17007725. Epub 2017 Jun 17.
5
Cloning, expression, purification, crystallization and preliminary crystallographic analysis of the kinase domain of AtMAP4Kalpha2 from Arabidopsis thaliana.拟南芥AtMAP4Kalpha2激酶结构域的克隆、表达、纯化、结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Jul;69(Pt 7):788-91. doi: 10.1107/S174430911301525X. Epub 2013 Jun 28.
6
Crystallization and preliminary crystallographic analysis of recombinant VSP1 from Arabidopsis thaliana.拟南芥重组VSP1的结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Feb 1;66(Pt 2):201-3. doi: 10.1107/S1744309109053688. Epub 2010 Jan 28.
7
Cloning, purification, crystallization and preliminary X-ray analysis of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana.拟南芥组氨酸激酶CKI1受体结构域的克隆、纯化、结晶及初步X射线分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 1;65(Pt 5):478-81. doi: 10.1107/S1744309109012032. Epub 2009 Apr 24.
8
Purification, crystallization and preliminary X-ray diffraction analysis of a cystathionine beta-synthase domain-containing protein, CDCP2, from Arabidopsis thaliana.来自拟南芥的含胱硫醚β-合酶结构域蛋白CDCP2的纯化、结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Sep 1;64(Pt 9):825-7. doi: 10.1107/S1744309108025128. Epub 2008 Aug 20.
9
SnRK2.6/OST1 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis of K50N and D160A mutants.拟南芥SnRK2.6/OST1：K50N和D160A突变体的克隆、表达、纯化、结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):364-8. doi: 10.1107/S1744309110053807. Epub 2011 Feb 25.
10
The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis.拟南芥中SOS3与SOS2调节结构域的复合物：克隆、表达、纯化、结晶及初步X射线分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt 7):568-70. doi: 10.1107/S174430910702533X. Epub 2007 Jun 11.
引用本文的文献
1
Functional implications of MIR domains in protein -mannosylation.MIR 结构域在蛋白质甘露糖基化中的功能意义。
Elife. 2020 Dec 24;9:e61189. doi: 10.7554/eLife.61189.
2
Structural characterization of binding mode of smoking cessation drugs to nicotinic acetylcholine receptors through study of ligand complexes with acetylcholine-binding protein.通过研究与乙酰胆碱结合蛋白的配体复合物，对戒烟药物与烟碱型乙酰胆碱受体结合模式的结构特征进行了表征。
J Biol Chem. 2012 Jul 6;287(28):23283-93. doi: 10.1074/jbc.M112.360347. Epub 2012 May 2.
3
Arabidopsis stromal-derived Factor2 (SDF2) is a crucial target of the unfolded protein response in the endoplasmic reticulum.拟南芥基质衍生因子 2（SDF2）是内质网未折叠蛋白反应的关键靶点。
J Biol Chem. 2010 Jun 4;285(23):18113-21. doi: 10.1074/jbc.M110.117176. Epub 2010 Apr 8.
本文引用的文献
1
Endoplasmic reticulum quality control and the unfolded protein response: insights from plants.内质网质量控制与未折叠蛋白反应：来自植物的见解
Traffic. 2008 Sep;9(10):1581-8. doi: 10.1111/j.1600-0854.2008.00780.x. Epub 2008 Jun 28.
2
Signal integration in the endoplasmic reticulum unfolded protein response.内质网未折叠蛋白反应中的信号整合
Nat Rev Mol Cell Biol. 2007 Jul;8(7):519-29. doi: 10.1038/nrm2199.
3
Gene expression in response to endoplasmic reticulum stress in Arabidopsis thaliana.拟南芥中内质网应激反应的基因表达
FEBS J. 2005 Jul;272(13):3461-76. doi: 10.1111/j.1742-4658.2005.04770.x.
4
Crystal structure of the ligand binding suppressor domain of type 1 inositol 1,4,5-trisphosphate receptor.1型肌醇1,4,5-三磷酸受体配体结合抑制结构域的晶体结构
Mol Cell. 2005 Jan 21;17(2):193-203. doi: 10.1016/j.molcel.2004.11.047.
5
Characterization of pancreatic ERj3p, a homolog of yeast DnaJ-like protein Scj1p.胰腺内质网蛋白3（ERj3p）的特性研究，它是酵母类DnaJ蛋白Scj1p的同源物。
Biol Chem. 2004 May;385(5):389-95. doi: 10.1515/BC.2004.043.
6
Genomic analysis of the unfolded protein response in Arabidopsis shows its connection to important cellular processes.拟南芥中未折叠蛋白反应的基因组分析表明其与重要细胞过程的联系。
Plant Cell. 2003 Feb;15(2):561-76. doi: 10.1105/tpc.007609.
7
A subset of chaperones and folding enzymes form multiprotein complexes in endoplasmic reticulum to bind nascent proteins.一部分伴侣蛋白和折叠酶在内质网中形成多蛋白复合物，以结合新生蛋白质。
Mol Biol Cell. 2002 Dec;13(12):4456-69. doi: 10.1091/mbc.e02-05-0311.
8
Structure of the inositol 1,4,5-trisphosphate receptor binding core in complex with its ligand.与配体结合的肌醇1,4,5-三磷酸受体结合核心的结构。
Nature. 2002 Dec 12;420(6916):696-700. doi: 10.1038/nature01268. Epub 2002 Nov 17.
9
Murine and human SDF2L1 is an endoplasmic reticulum stress-inducible gene and encodes a new member of the Pmt/rt protein family.小鼠和人类的SDF2L1是一种内质网应激诱导基因，编码Pmt/rt蛋白家族的一个新成员。
Biochem Biophys Res Commun. 2001 Jan 12;280(1):407-14. doi: 10.1006/bbrc.2000.4111.
10
Novel repeats in ryanodine and IP3 receptors and protein O-mannosyltransferases.雷诺丁受体和三磷酸肌醇受体以及蛋白质O-甘露糖基转移酶中的新型重复序列。
Trends Biochem Sci. 2000 Feb;25(2):48-50. doi: 10.1016/s0968-0004(99)01513-3.
Zotero 插件