Department of Dental Materials and Prosthodontics, School of Dentistry, São Paulo State University (Unesp), Araraquara, São Paulo, Brazil.
Department of Organic Chemistry, Institute of Chemistry, São Paulo State University (Unesp), São Paulo, Araraquara, Brazil.
Arch Microbiol. 2022 Aug 20;204(9):569. doi: 10.1007/s00203-022-03177-1.
The objective of this study was evaluate, in vivo model, the antifungal activity of Cryptocarya moschata extract against Candida albicans and its biocompatibility. The animals (N = 50) were divided into groups (n = 5): CI/CG: candidiasis was induced and treated with C. moschata extract (0.045 g/mL); CI/NG: candidiasis was induced and treated with nystatin; CI/NT: candidiasis was induced and no treated; CI/CG-2: candidiasis was induced and treated with C. moschata extract (0.045 g/mL), reapplied after 24 h; CI/NG-2: candidiasis was induced and treated with nystatin, reapplied after 24 h; NCI/NT: candidiasis was not induced and no treated; NCI/CG: candidiasis was not induced and treated with C. moschata extract (0.045 g/mL); NCI/NG: candidiasis was not induced treated with nystatin; NCI/CG-2: candidiasis was not induced and treated with C. moschata extract (0.045 g/mL), reapplied after 24 h; NCI/NG-2: candidiasis was not induced and treated with nystatin, reapplied after 24 h. The fungi present in the lingual dorsum of mice were collected and analyzed by the count of colony-forming units. In addition, histological analysis was performed. Histologically, there was no cell damage in the mice's tongue, and there was a decrease in Candida biofilm, similar to the use of nystatin. It was concluded that the C. moschata extract was effective against C. albicans and was biocompatible.
本研究旨在体内模型中评估Cryptocarya moschata 提取物对白色念珠菌的抗真菌活性及其生物相容性。将动物（N=50）分为以下几组（n=5）：CI/CG：诱导念珠菌病并用 C. moschata 提取物（0.045 g/mL）治疗；CI/NG：诱导念珠菌病并用制霉菌素治疗；CI/NT：诱导念珠菌病但不治疗；CI/CG-2：诱导念珠菌病并用 C. moschata 提取物（0.045 g/mL）治疗，24 小时后再次应用；CI/NG-2：诱导念珠菌病并用制霉菌素治疗，24 小时后再次应用；NCI/NT：未诱导念珠菌病且未治疗；NCI/CG：未诱导念珠菌病并用 C. moschata 提取物（0.045 g/mL）治疗；NCI/NG：未诱导念珠菌病并用制霉菌素治疗；NCI/CG-2：未诱导念珠菌病并用 C. moschata 提取物（0.045 g/mL）治疗，24 小时后再次应用；NCI/NG-2：未诱导念珠菌病并用制霉菌素治疗，24 小时后再次应用。收集小鼠舌背的真菌并通过菌落形成单位计数进行分析。此外，还进行了组织学分析。组织学上，小鼠舌细胞没有损伤，且念珠菌生物膜减少，类似于制霉菌素的作用。因此，C. moschata 提取物对白色念珠菌有效且具有生物相容性。
