Martins Joyce da Silva, Junqueira Juliana Campos, Faria Raquel Lourdes, Santiago Naiara Fonseca, Rossoni Rodnei Dennis, Colombo Carlos Eduardo Dias, Jorge Antonio Olavo Cardoso
Department of Biosciences and Oral Diagnosis, School of Dentistry of São José dos Campos, São Paulo State University-UNESP, São Paulo, Brazil.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Jan;111(1):71-7. doi: 10.1016/j.tripleo.2010.08.012.
This study evaluated the effects of photodynamic therapy on pathogenicity of Candida albicans.
Fifty-six rats were submitted to development of candidiasis on the tongue dorsum by C. albicans inoculations. After 5 days, different treatments were administered: laser and photosynthesizer methylene blue (L+P+); laser only (L+P-); photosensitizer only (L-P+); and physiologic solution only (L-P-). Samples of the oral cavity were collected for a count of colony-forming units per mL. Colonies were isolated for evaluation of proteinase and phospholipase activities. The rats were killed for microscopic analysis of the tongue dorsum. The data were analyzed by analysis of variance, Kruskal-Wallis, and Bonferroni tests.
The number of C. albicans recovered from the oral cavity of the rats was similar between the groups (P = .106). The L+P+ group showed fewer microscopic lesions of candidiasis than the L-P- group (P = .001). The L+P+ group presented lower proteinase activity compared with the other groups, with significant difference between the groups L+P+ and L-P+ (P = .018).
Photodynamic therapy reduced the microscopic lesions of experimental candidiasis in rats and inhibited the proteinase activity of C. albicans.
本研究评估光动力疗法对白色念珠菌致病性的影响。
56只大鼠通过接种白色念珠菌在舌背诱发念珠菌病。5天后，给予不同治疗：激光与光合剂亚甲蓝（L+P+）；仅激光（L+P-）；仅光敏剂（L-P+）；以及仅生理溶液（L-P-）。采集口腔样本以计数每毫升的菌落形成单位。分离菌落以评估蛋白酶和磷脂酶活性。处死大鼠以对舌背进行显微镜分析。数据通过方差分析、Kruskal-Wallis检验和Bonferroni检验进行分析。
各组大鼠口腔中回收的白色念珠菌数量相似（P = 0.106）。L+P+组的念珠菌病微观病变比L-P-组少（P = 0.001）。与其他组相比，L+P+组的蛋白酶活性较低，L+P+组和L-P+组之间存在显著差异（P = 0.018）。
光动力疗法减少了大鼠实验性念珠菌病的微观病变，并抑制了白色念珠菌的蛋白酶活性。
