Senel Figen Cizmeci, Kadioglu Duman Mine, Muci Efnan, Cankaya Mustafa, Pampu A Alper, Ersoz Safak, Gunhan Omer
Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Karadeniz Technical University, Trabzon, Turkey.
Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010 Mar;109(3):385-91. doi: 10.1016/j.tripleo.2009.10.011. Epub 2010 Jan 8.
The aim of this study was to histopathologically evaluate the effects of pamidronate and zoledronate on the mandible in an animal model.
Sixty female Sprague-Dawley rats were used in this study. Animals were divided into 6 groups (10 per group): control-1 (C1), injected with saline solution for 6 weeks; zoledronate-1 (ZA1), injected with zoledronate for 6 weeks; pamidronate-1 (PA1), injected with pamidronate for 6 weeks; control-2 (C2), injected with saline solution for 8 weeks; zoledronate-2 (ZA2), injected with zoledronate for 8 weeks; and pamidronate-2 (PA2), injected with pamidronate for 8 weeks. No dental procedures were performed on the animals. Rats were killed 2 days after the end of drug therapy, and the posterior and anterior mandible and femur of each rat were evaluated histopathologically.
Histological examination revealed inflammation limited to the posterior mandible of the ZA2 and PA2 groups; the anterior mandible and femur were not affected. Soft tissue necrosis was evident in one rat in the ZA2 group.
Specific, bisphosphonate-associated inflammatory bony and soft tissue changes were observed in the mandible, suggesting that these drugs may set the stage for altered healing associated with the development of bisphosphonate-related osteonecrosis of the jaw.
本研究旨在通过组织病理学方法评估帕米膦酸盐和唑来膦酸盐对动物模型下颌骨的影响。
本研究使用了60只雌性斯普拉格-道利大鼠。动物被分为6组（每组10只）：对照组-1（C1），注射生理盐水6周；唑来膦酸盐-1（ZA1），注射唑来膦酸盐6周；帕米膦酸盐-1（PA1），注射帕米膦酸盐6周；对照组-2（C2），注射生理盐水8周；唑来膦酸盐-2（ZA2），注射唑来膦酸盐8周；帕米膦酸盐-2（PA2），注射帕米膦酸盐8周。未对动物进行牙科手术。在药物治疗结束后2天处死大鼠，对每只大鼠的下颌骨前后部和股骨进行组织病理学评估。
组织学检查显示，炎症仅限于ZA2组和PA2组的下颌骨后部；下颌骨前部和股骨未受影响。ZA2组有一只大鼠出现明显的软组织坏死。
在下颌骨中观察到了与双膦酸盐相关的特定炎性骨和软组织变化，这表明这些药物可能为与双膦酸盐相关的颌骨坏死发展相关的愈合改变奠定了基础。
