Department of Science of Physical Function, Division of Maxillofacial Surgery, Kyushu Dental College, Kitakyushu, Japan.
Int J Oral Maxillofac Surg. 2013 Nov;42(11):1481-7. doi: 10.1016/j.ijom.2013.06.011. Epub 2013 Aug 6.
Clinically, bisphosphonate-related osteonecrosis (BRON) only occurs in the jaw (BRONJ). We aimed to determine differences between the jaw and other bones, as well as the relationship between periodontal pathogens and BRON. Twelve male Wistar rats were divided into two groups: group Z (n=6) were subcutaneously injected with zoledronic acid weekly for 4 weeks; group C (n=6) were injected with saline. One week after the final injection, rats in both groups were divided into three subgroups: Aa subgroup was injected with freeze-dried Aggregatibacter actinomycetemcomitans into bone marrow of the mandibles and femurs, while CFA and saline subgroups were injected with complete Freund's adjuvant (CFA) or saline using the same protocol. Four weeks after those injections, the rats were euthanized. Areas of osteonecrosis were measured histologically. Statistically, rats in group Z showed significantly wider osteonecrosis areas in both mandibles and femurs with each type of local injection than rats in group C. In group Z, mandibles and femurs stimulated with A. actinomycetemcomitans or CFA had significantly wider osteonecrosis areas than those stimulated with saline. We have developed a rat model with BRONJ-like lesions. Our results showed BRON to develop not only in the jaw but also in other types of bone following an inflammatory stimulus.
临床上，双膦酸盐相关性骨坏死（BRON）仅发生在颌骨（BRONJ）。我们旨在确定颌骨与其他骨骼之间的差异，以及牙周病原体与 BRON 之间的关系。将 12 只雄性 Wistar 大鼠分为两组：唑来膦酸组（n=6）每周皮下注射唑来膦酸 4 周；生理盐水组（n=6）注射生理盐水。末次注射后 1 周，两组大鼠均分为三组：Aa 亚组向颌骨和股骨骨髓内注射冻干伴放线放线杆菌，CFA 和生理盐水亚组则采用相同方案注射完全弗氏佐剂（CFA）或生理盐水。注射后 4 周，处死大鼠。组织学测量骨坏死面积。统计学分析显示，与生理盐水组相比，唑来膦酸组各局部注射的颌骨和股骨的骨坏死面积明显更宽。在唑来膦酸组中，用 A. actinomycetemcomitans 或 CFA 刺激的颌骨和股骨的骨坏死面积明显大于用生理盐水刺激的骨坏死面积。我们已经建立了一种具有 BRONJ 样病变的大鼠模型。我们的结果表明，BRON 不仅在颌骨中发展，而且在炎症刺激后也在其他类型的骨骼中发展。
