Division of Nephrology, Department of Internal Medicine, Marmara University School of Medicine, Tophanelioglu Caddesi, No 15/10, Altunizade, Uskudar, Istanbul 32622, Turkey.
Eur J Endocrinol. 2010 Apr;162(4):711-7. doi: 10.1530/EJE-09-0795. Epub 2010 Jan 8.
Circulating endothelial cells (CECs) have emerged as vascular damage markers and are increased in type 2 diabetic patients. Since type 1 diabetes is associated with vascular damage, we hypothesized high CEC numbers in this patient population.
Thirty-nine patients with type 1 diabetes and 39 controls were included. CECs were isolated using anti-CD146-coated Dynabeads, stained with Ulex lectin-1, and counted by fluorescence microscopy. Endothelial function was measured as flow-mediated dilation (FMD). Thiobarbituric acid reactive substances (TBARS), total glutathione levels (GSH), and paraoxonase (PON) activity levels were measured as oxidative stress markers.
Patients with type 1 diabetes mellitus had higher number of CECs (7.46+/-5.37 vs 2.13+/-1.13 cells/ml, P<0.001), lower FMD (7.87+/-2.19 vs 12.06+/-2.34%, P<0.001), higher TBARS (4.94+/-1.20 vs 3.07+/-0.75 nmol/MDA, P<0.001), lower GSH (206.12+/-98.06 vs 353.61+/-68.45 microM, P<0.001), and lower PON activity levels (89.10+/-17.82 vs 127.65+/-29.01 U/l, P<0.001) as compared to controls. There was positive correlation between CEC numbers and HbAlc levels (r=0.49, P=0.002). CECs and fasting glucose levels were not correlated. There was no correlation between the number of CECs and FMD. Furthermore, there were no correlations between the number of CECs and TBARS, GSH and PON activity levels. Multiple regression analysis showed that HbAlc levels (r(2)=0.40, P<0.009) were associated with CEC numbers.
CECs are elevated in patients with type 1 diabetes mellitus reflecting endothelial damage. This increase is dependent on long-term glucose control.
循环内皮细胞（CEC）已成为血管损伤的标志物，在 2 型糖尿病患者中增加。由于 1 型糖尿病与血管损伤有关，我们假设这种患者人群中 CEC 数量较高。
纳入 39 名 1 型糖尿病患者和 39 名对照者。使用抗 CD146 包被的 Dynabeads 分离 CEC，用 Ulex lectin-1 染色，通过荧光显微镜计数。通过血流介导的扩张（FMD）测量内皮功能。测量丙二醛（TBARS）、总谷胱甘肽水平（GSH）和对氧磷酶（PON）活性水平作为氧化应激标志物。
1 型糖尿病患者的 CEC 数量较高（7.46+/-5.37 与 2.13+/-1.13 个/ml，P<0.001），FMD 较低（7.87+/-2.19 与 12.06+/-2.34%，P<0.001），TBARS 较高（4.94+/-1.20 与 3.07+/-0.75 nmol/MDA，P<0.001），GSH 水平较低（206.12+/-98.06 与 353.61+/-68.45 microM，P<0.001），PON 活性水平较低（89.10+/-17.82 与 127.65+/-29.01 U/l，P<0.001）。与对照组相比，CEC 数量与 HbAlc 水平呈正相关（r=0.49，P=0.002）。CEC 与空腹血糖水平不相关。CEC 数量与 FMD 之间没有相关性。此外，CEC 数量与 TBARS、GSH 和 PON 活性水平之间没有相关性。多元回归分析显示，HbAlc 水平（r(2)=0.40，P<0.009）与 CEC 数量相关。
1 型糖尿病患者的 CEC 升高，反映内皮损伤。这种增加取决于长期血糖控制。
