叶下珠素对乙醇处理的大鼠原代肝细胞的保护作用。
Protective activity of phyllanthin in ethanol-treated primary culture of rat hepatocytes.
机构信息
Pharmacological Action of Natural Products Research Unit, Department of Pharmacology and Physiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, PhyaThai Road, Pathumwan, Bangkok 10330, Thailand.
出版信息
J Ethnopharmacol. 2010 Mar 2;128(1):172-6. doi: 10.1016/j.jep.2010.01.003. Epub 2010 Jan 12.
AIM OF THE STUDY
To investigate the protective effect of phyllanthin (a known principal constituent of Phyllanthus amarus Schum. et Thonn.) on ethanol-induced rat liver cell injury.
MATERIALS AND METHODS
Primary culture of rat hepatocytes (24h culturing) were pretreated with phyllanthin (1, 2, 3 and 4 microg/ml) for 24h. After 24h pretreatment, cells were treated with ethanol (80 microl/ml) for 2h.
RESULTS
Ethanol decreased %MTT, increased the release of transaminases (ALT and AST) with the increase in the production of intracellular ROS and lipid peroxidation. Phyllanthin demonstrated its role in protection by antagonizing the above effect induced by ethanol. Phyllanthin also restored the antioxidant capability of rat hepatocytes including level of total glutathione, and activities of superoxide dismutase (SOD) and glutathione reductase (GR) which were reduced by ethanol.
CONCLUSIONS
These results suggested the hepatoprotective effect of phyllanthin against ethanol-induced oxidative stress causing rat liver cell damage through its antioxidant activity.
研究目的
研究叶下珠素（叶下珠的已知主要成分）对乙醇诱导的大鼠肝细胞损伤的保护作用。
材料和方法
原代培养的大鼠肝细胞（培养 24 小时）用叶下珠素（1、2、3 和 4μg/ml）预处理 24 小时。预处理 24 小时后，用乙醇（80μl/ml）处理细胞 2 小时。
结果
乙醇降低了%MTT，增加了转氨酶（ALT 和 AST）的释放，同时增加了细胞内 ROS 和脂质过氧化的产生。叶下珠素通过拮抗乙醇诱导的上述作用，显示出其保护作用。叶下珠素还恢复了乙醇降低的大鼠肝细胞的抗氧化能力，包括总谷胱甘肽水平，以及超氧化物歧化酶（SOD）和谷胱甘肽还原酶（GR）的活性。
结论
这些结果表明，叶下珠素通过其抗氧化活性对乙醇诱导的氧化应激引起的大鼠肝损伤具有保护作用。
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