Center for Radiological Research, Columbia University Medical Center, New York, NY 10032, USA.
Health Phys. 2010 Feb;98(2):209-17. doi: 10.1097/HP.0b013e3181ab3cb6.
In response to the recognized need for high throughput biodosimetry methods for use after large-scale radiological events, a logical approach is complete automation of standard biodosimetric assays that are currently performed manually. The authors describe progress to date on the RABIT (Rapid Automated BIodosimetry Tool), designed to score micronuclei or gamma-H2AX fluorescence in lymphocytes derived from a single drop of blood from a fingerstick. The RABIT system is designed to be completely automated, from the input of the capillary blood sample into the machine to the output of a dose estimate. Improvements in throughput are achieved through use of a single drop of blood, optimization of the biological protocols for in situ analysis in multi-well plates, implementation of robotic-plate and liquid handling, and new developments in high-speed imaging. Automating well-established bioassays represents a promising approach to high-throughput radiation biodosimetry, both because high throughputs can be achieved, but also because the time to deployment is potentially much shorter than for a new biological assay. Here the authors describe the development of each of the individual modules of the RABIT system and show preliminary data from key modules. System integration is ongoing, followed by calibration and validation.
为响应在大规模放射性事件后使用高通量生物剂量测定方法的公认需求，一种合理的方法是完全自动化目前手动进行的标准生物剂量测定测定。作者描述了迄今为止在 RABIT（快速自动生物剂量测定工具）方面的进展，该工具旨在对来自手指刺血的单个血滴中的淋巴细胞中的微核或γ-H2AX 荧光进行评分。RABIT 系统旨在完全自动化，从毛细血管血样输入机器到剂量估计的输出。通过使用一滴血、优化用于多孔板中原位分析的生物协议、实施机器人板和液体处理以及高速成像的新发展，实现了高通量的提高。自动化成熟的生物测定代表了高通量辐射生物剂量测定的一种有前途的方法，这不仅是因为可以实现高通量，而且因为部署的时间可能比新的生物测定短得多。在这里，作者描述了 RABIT 系统的每个单独模块的开发，并展示了关键模块的初步数据。系统集成正在进行中，随后是校准和验证。
