Department of Food and Nutrition, Obesity/Diabetes Center, College of Science, Natural Hoseo University, Asan 336-795, Korea.
J Agric Food Chem. 2010 Feb 10;58(3):1551-7. doi: 10.1021/jf903432b.
Glyceollins are a category of phytoalexins that are produced by soybeans under fungal stress, but their effects on glucose homeostasis remain unknown. We hypothesized that glyceollins play an important role in glucose homeostasis by regulating glucose utilization in adipocytes and improving beta-cell function and survival. Glyceollins improved insulin-stimulated glucose uptake in 3T3-L1 adipocytes without activating the peroxisome proliferator-activated receptor-gamma agonist. They decreased triacylglycerol accumulation in adipocytes. In addition, glyceollins slightly improved glucose-stimulated insulin secretion without palmitate treatment in Min6 cells, and they potentiated insulinotropic actions when 500 microM palmitate was used to induce beta-cell dysfunction. This was associated with decreased beta-cell apoptosis because of the attenuation of endoplasmic reticulum stress, as determined by mRNA levels of XBP-1, ATF-4, ATF-6, and CHOP. Glyceollins also potentiated GLP-1 secretion to enhance insulinotropic actions in enteroendocrine cells. In conclusion, glyceollins help normalize glucose homeostasis by potentiating beta-cell function and survival and improving glucose utilization in adipocytes.
大豆在真菌胁迫下产生的glyceollins 是一类植物抗毒素，但它们对葡萄糖稳态的影响尚不清楚。我们假设 glyceollins 通过调节脂肪细胞中的葡萄糖利用以及改善β细胞功能和存活，在葡萄糖稳态中发挥重要作用。glyceollins 在不激活过氧化物酶体增殖物激活受体-γ激动剂的情况下，增加了 3T3-L1 脂肪细胞中胰岛素刺激的葡萄糖摄取。它们减少了脂肪细胞中的三酰甘油积累。此外，glyceollins 在 Min6 细胞中，即使没有棕榈酸处理，也能稍微改善葡萄糖刺激的胰岛素分泌，并在使用 500μM 棕榈酸诱导β细胞功能障碍时增强胰岛素促分泌作用。这与β细胞凋亡的减少有关，因为内质网应激的减弱，通过 XBP-1、ATF-4、ATF-6 和 CHOP 的 mRNA 水平来确定。glyceollins 还增强了 GLP-1 的分泌，以增强肠内分泌细胞中的胰岛素促分泌作用。总之，glyceollins 通过增强β细胞功能和存活以及改善脂肪细胞中的葡萄糖利用，有助于使葡萄糖稳态正常化。
