Lopes Murilo Baena, Sinhoreti Mário A C, Gonini Júnior Alcides, Consani Simonides, McCabe John F
Department of Dentistry, Dental School, University of Northern Paraná, Londrina, PR, Brazil.
Braz Dent J. 2009;20(4):279-83. doi: 10.1590/s0103-64402009000400003.
This study compared the tubular dimensions and distribution of human and bovine dentin. Ten human molars and 10 bovine incisors were ground with a high-speed handpiece to obtain 3 sections at different dentin depths (superficial, middle and deep). The specimens were sputter-coated with gold to be examined under scanning electron microscopy (SEM). Three SEM micrographs were recorded randomly for each dentin depth. The number of tubules was counted and the diameter of 5 tubules selected at random was measured in each SEM micrograph. Data were analyzed statistically by ANOVA and Tukey's test (alpha=0.05). In bovine teeth, superficial dentin (4.21 microm) and middle dentin (3.98 microm) had a significantly greater (p<0.05) diameter than deep dentin (3.14 microm) tubules. In human teeth, superficial dentin tubule diameter (2.42 microm) was significantly smaller (p<0.05) than deep dentin (2.99 microm) and middle dentin (2.94 microm) tubule diameters, which did not differ significantly from each other (p>0.05). The number of tubules per square millimeter, regardless of the region, was significantly greater in human dentin (22,329) than in bovine dentin (15,964). There was a clear difference in tubule structure and morphology between human and bovine dentin.
本研究比较了人类和牛牙本质的小管尺寸及分布。使用高速手机研磨10颗人类磨牙和10颗牛切牙，以获取不同牙本质深度（表层、中层和深层）的3个切片。将标本进行金溅射镀膜，以便在扫描电子显微镜（SEM）下检查。对每个牙本质深度随机记录3张SEM显微照片。在每张SEM显微照片中计数小管数量，并随机选择5个小管测量其直径。数据采用方差分析和Tukey检验进行统计学分析（α = 0.05）。在牛牙中，表层牙本质（4.21微米）和中层牙本质（3.98微米）的小管直径显著大于深层牙本质（3.14微米）（p < 0.05）。在人类牙齿中，表层牙本质小管直径（2.42微米）显著小于深层牙本质（2.99微米）和中层牙本质（2.94微米）的小管直径，而后两者之间无显著差异（p > 0.05）。无论区域如何，每平方毫米的小管数量在人类牙本质（22,329个）中显著多于牛牙本质（15,964个）。人类和牛牙本质的小管结构和形态存在明显差异。
