Wang Yifan, Chen Shangsi, Zhang Minjun, Chen Lili, Zhou Chenchen, Tan Shenglong
School of Stomatology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
Hubei Province Key Laboratory of Oral and Maxillofacial Development and Regeneration, Wuhan 430022, China.
iScience. 2024 Nov 23;27(12):111474. doi: 10.1016/j.isci.2024.111474. eCollection 2024 Dec 20.
Teeth undergo continuous demineralization and remineralization influenced by dietary acid and saliva. Excessive dietary acid attack disrupts this balance, exposing dentin tubules and causing dental hypersensitivity (DH). Due to low acid resistance, traditional anti-DH regents such as calcium phosphate minerals fail in long-term occlusion of dentin tubules, resulting in recurrent attacks of DH. Hence, we fabricate nano hydroxyapatite (nHA)-silica (nHASi) with a core-shell structure that can not only fill in the dentin tubules, releasing Ca and PO from the nHA core for biomineralization, but also exhibit remarkable acid resistance due to the silica shell. Our study demonstrates a continuous growth of hydroxyapatite (HA) nanocrystals within nHASi during cyclic de/remineralization. When applied with toothpaste, nHASi gradually enhances dentin tubule occlusion over de/remineralization cycles. Additionally, extracts of nHASi exhibit excellent cytocompatibility and odontogenic inductivity . This work provides a paradigm for developing effective anti-allergic materials for the long-term management of DH.
牙齿会受到饮食中的酸和唾液的影响，持续发生脱矿和再矿化。过多的饮食酸侵蚀会破坏这种平衡，使牙本质小管暴露并导致牙齿敏感症（DH）。由于耐酸性低，传统的抗牙齿敏感剂如磷酸钙矿物质无法长期封闭牙本质小管，导致牙齿敏感症反复发作。因此，我们制备了具有核壳结构的纳米羟基磷灰石（nHA）-二氧化硅（nHASi），它不仅可以填充牙本质小管，从nHA核中释放钙和磷用于生物矿化，而且由于二氧化硅壳还具有显著的耐酸性。我们的研究表明，在循环脱矿/再矿化过程中，nHASi内的羟基磷灰石（HA）纳米晶体持续生长。当与牙膏一起使用时，nHASi在脱矿/再矿化循环中逐渐增强牙本质小管的封闭。此外，nHASi提取物具有优异的细胞相容性和牙源性诱导性。这项工作为开发用于牙齿敏感症长期管理的有效抗过敏材料提供了范例。
