Pathology Department, Health Sciences Center, Federal University of Santa Catarina, Florianopolis.
J Oral Pathol Med. 2010 Mar;39(3):230-5. doi: 10.1111/j.1600-0714.2009.00846.x. Epub 2010 Jan 11.
Altered expression of extracellular matrix (ECM) components has been reported in several pathologies; however, few ECM proteins have been evaluated in adenomatoid odontogenic tumor (AOT). The aim of this study was to analyze the expression and distribution of the ECM proteoglycans: biglycan and decorin; and glycoproteins: osteonectin, osteopontin, bone sialoprotein and osteocalcin in the AOT. Three-micrometer sections from paraffin-embedded specimens were evaluated employing a streptavidin-biotin immunohistochemical method with the antibodies against the proteins previously cited. Only the osteonectin was expressed in the epithelial cells. The eosinophilic amorphous material and the connective tissue showed expression of all components studied. The calcification foci expressed only osteopontin. In conclusion, the low expression of the components studied in neoplastic epithelial cells suggests that the epithelial cells act probably as stimulators of the expression by the stroma, which in turn can act as agonist or antagonist of the tumor growth. These results suggest that the components studied probably have a key role in the biological behavior of the AOT.
细胞外基质（ECM）成分的表达改变已在几种病理中报道；然而，在腺牙源性肿瘤（AOT）中仅少数 ECM 蛋白被评估过。本研究的目的是分析 ECM 蛋白聚糖：核心蛋白聚糖和聚集蛋白聚糖；以及糖蛋白：骨桥蛋白、骨连接素、骨唾液蛋白和骨钙素在 AOT 中的表达和分布。使用针对上述蛋白的链霉亲和素-生物素免疫组织化学方法，评估来自石蜡包埋标本的 3μm 切片。仅在上皮细胞中表达骨连接素。嗜酸性无定形物质和结缔组织表达了所有研究的成分。钙化灶仅表达骨桥蛋白。总之，研究的成分在上皮肿瘤细胞中的低表达表明上皮细胞可能作为基质表达的刺激物，而基质反过来又可以作为肿瘤生长的激动剂或拮抗剂。这些结果表明，研究的成分可能在 AOT 的生物学行为中起关键作用。
