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蛋白A/G在四种动物弓形虫感染血清学诊断酶联免疫吸附试验中的应用。

Use of protein AG in an enzyme-linked immunosorbent assay for serodiagnosis of Toxoplasma gondii infection in four species of animals.

作者信息

Zhang Donglin, Wang Zhengsong, Fang Rui, Nie Hao, Feng Huihui, Zhou Yanqin, Zhao Junlong

机构信息

State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, Hubei, People's Republic of China.

出版信息

Clin Vaccine Immunol. 2010 Mar;17(3):485-6. doi: 10.1128/CVI.00479-09. Epub 2010 Jan 13.

Abstract

An enzyme-linked immunosorbent assay (ELISA) employing protein AG (AG-ELISA) as a conjugate was developed to detect anti-Toxoplasma gondii antibodies (Ab) in experimentally infected pigs and naturally infected pigs, goats, dogs, and cats. The results indicate that AG-ELISA can be a useful method for serological diagnosis of T. gondii infection in these four species of animals.

摘要

开发了一种以蛋白A/G(AG-ELISA)作为结合物的酶联免疫吸附测定法,用于检测实验感染猪以及自然感染猪、山羊、狗和猫体内的抗弓形虫抗体(Ab)。结果表明,AG-ELISA可能是这四种动物弓形虫感染血清学诊断的一种有用方法。

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