Liu Ying, Zhou Jianliang, Li Ping
Key Laboratory of Modern Chinese Medicines, Ministry of Education, Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210009, China.
Se Pu. 2009 Sep;27(5):682-9.
The analysis of chemical components of Chinese herbal medicines (CHMs) is one of the most critical issues not only for screening and analyzing the bioactive components but also for controlling their quality. However, due to the complexity of the chemical constituents of CHMs, it is difficult to separate them on column within a short time. In the recent, the fast and ultra performance liquid chromatography, including ultra high pressure liquid chromatography, high performance liquid chromatography based on the monolithic columns and high temperature liquid chromatography, are of particular interest because of the high resolution and fast analytical speed provided by these techniques. This overview covers the principle and separation characteristics of these techniques, as well as their applications in Chinese herbal medicines.
中药化学成分分析不仅是筛选和分析生物活性成分的关键问题之一，也是控制其质量的关键问题之一。然而，由于中药化学成分的复杂性，很难在短时间内在柱上对其进行分离。近年来，快速和超高效液相色谱法，包括超高压液相色谱法、基于整体柱的高效液相色谱法和高温液相色谱法，因其提供的高分辨率和快速分析速度而备受关注。本综述涵盖了这些技术的原理、分离特性及其在中药中的应用。
