Department of Biology, School of Farmacy, San Pablo CEU University, PO Box 67, Boadilla del Monte, 28668 Madrid, Spain.
J Agric Food Chem. 2010 Feb 10;58(3):1484-92. doi: 10.1021/jf903299a.
Nine plant growth-promoting rhizobacteria from different backgrounds were assayed on Glycine max var. Osumi to evaluate their potential as biotic elicitors to increase isoflavone (IF) levels. Strains were inoculated on 2 day old pregerminated seeds. Six days after inoculation, the seedlings were harvested. Biometric parameters were registered, and IFs were determined. Although only one strain (N21.4) increased total IF contents and only one (M84) caused significant decreases in total IF, five different behaviors were detected when the daidzein and genistein families were analyzed separately. All strains triggered IF metabolism so further studies have to be developed since the different beneficial effects of IF through the diet may be due to the different IF profiles. These are encouraging results from two points of view: (1) N21.4 increases IF in seedlings, and (2) all other beneficial strains trigger IF metabolism differentially; hence, both facts could be used to prepare food supplements or as enriched standardized foods after full development of the biotechnological procedure.
从不同背景中选取了 9 株植物促生根际细菌，用于评估其作为生物诱导子增加异黄酮 (IF) 水平的潜力。将菌株接种于 2 日龄预发芽种子上。接种后 6 天，收获幼苗。记录生物计量参数并测定 IF。虽然只有一株菌（N21.4）增加了总 IF 含量，只有一株菌（M84）导致总 IF 显著减少，但当分别分析大豆苷元和染料木黄酮家族时，检测到了 5 种不同的行为。所有菌株都触发了 IF 代谢，因此需要进一步研究，因为通过饮食获得的不同 IF 有益效果可能是由于不同的 IF 谱。这从两个方面来看是令人鼓舞的结果：（1）N21.4 增加了幼苗中的 IF，（2）所有其他有益菌株都以不同的方式触发 IF 代谢；因此，这两个事实都可以用于制备食品补充剂，或在生物技术程序完全开发后作为富化标准化食品。
