Universidad CEU San Pablo, Facultad de Farmacia, Boadilla del Monte, Madrid, Spain.
Plant Foods Hum Nutr. 2013 Sep;68(3):299-305. doi: 10.1007/s11130-013-0373-x.
The effect of two bacterial strains to enhance bioactive contents (total phenolic compounds, total flavonoid compounds and isoflavones) and antioxidant activity on 3-day-old soybean sprouts were investigated. To identify bacterial determinants responsible for these effects, viable and UV-treated strains were delivered to wounded seeds at different concentration. Multivariate analysis performed with all the evaluated parameters indicated the different effectiveness of Stenotrophomonas maltophilia N5.18 and Pseudomonas fluorescens N21.4 based on different structural and metabolic determinants for each. N21.4 increased total phenolics and isoflavones from the genistein family, while N5.18 triggered biosynthesis of daidzein and genistein families coupled to a decrease in total phenolics, suggesting different molecular targets in the phenilpropanoid pathway. Only extracts from N5.18 treated seeds showed an improved antioxidant activity according to the β-carotene bleaching prevention method. In summary, bioeffectors from both bacterial strains are effective tools to improve soybean sprouts quality; structural elicitors from N5.18 also enhanced antioxidant activity, being the best alternative for further development of a biotechnological procedure.
研究了两种细菌菌株对 3 日龄大豆芽中生物活性成分（总酚化合物、总类黄酮化合物和异黄酮）和抗氧化活性的影响。为了确定这些影响的细菌决定因素，将活菌和紫外线处理的菌株以不同浓度递送到受伤的种子上。对所有评估参数进行的多元分析表明，基于每种菌株不同的结构和代谢决定因素，嗜麦芽寡养单胞菌 N5.18 和荧光假单胞菌 N21.4 的效果不同。N21.4 增加了来自染料木黄酮家族的总酚类化合物和异黄酮，而 N5.18 触发了大豆苷元和染料木黄酮家族的生物合成，同时总酚类化合物减少，表明在苯丙素途径中有不同的分子靶标。只有来自 N5.18 处理种子的提取物根据β-胡萝卜素漂白抑制法显示出改善的抗氧化活性。总之，两种细菌菌株的生物效应物是改善大豆芽质量的有效工具；N5.18 的结构诱导剂还增强了抗氧化活性，是进一步开发生物技术程序的最佳选择。
