Dibart Serge, Warbington Martha, Su Ming Fan, Skobe Ziedonis
Department of Periodontology and Oral Biology, Boston University School of Dental Medicine, Boston, MA 02118, USA.
Int J Oral Maxillofac Implants. 2005 Sep-Oct;20(5):732-7.
To test in vitro whether the seal provided by the locking taper used in the implant-abutment connection was capable of preventing the invasion of oral microorganisms.
Twenty-five wide-body implants (5 x 11 mm) and 25 abutments were divided into 2 groups for a 2-phase experiment. The first phase tested the ability of the seal to shield the implant well from outside bacteria; the second phase tested the ability of the seal to prevent bacteria present in the implant well from seeping out. For phase 1, 10 implant-abutment units were immersed in a bacterial broth for 24 hours. The abutments were then separated from the implants and bacterial presence was evaluated using scanning electron microscopy. In phase 2, the tested abutments were inoculated with a droplet of soft agar bacterial gel and assembled with the implant. These units were incubated in a sterile nutrient broth for 72 hours, sampled, and plated to assess bacterial presence.
In phase 1, no bacteria were detected in any of the implant wells. In phase 2, no bacteria were detected in the nutrient broth or on the agar plates at 72 hours.
In implants where a microgap is present, microbial leakage could lead to inflammation and bone loss; thus, it is important to minimize bacterial presence in and around the the implant-abutment junction.
The seal provided by the locking taper design has been demonstrated to be hermetic with regard to bacterial invasion in vitro.
在体外测试种植体-基台连接中使用的锁定锥度所提供的密封是否能够防止口腔微生物的侵入。
将25个宽体种植体（5×11毫米）和25个基台分为两组进行两阶段实验。第一阶段测试密封对种植体免受外部细菌影响的能力；第二阶段测试密封防止种植体内存在的细菌渗出的能力。对于第一阶段，将10个种植体-基台单元浸入细菌培养液中24小时。然后将基台与种植体分离，并使用扫描电子显微镜评估细菌的存在情况。在第二阶段，将测试的基台接种一滴软琼脂细菌凝胶，并与种植体组装。将这些单元在无菌营养肉汤中孵育72小时，取样并接种以评估细菌的存在情况。
在第一阶段，任何种植体内均未检测到细菌。在第二阶段，72小时时在营养肉汤或琼脂平板上均未检测到细菌。
在存在微间隙的种植体中，微生物渗漏可能导致炎症和骨质流失；因此，尽量减少种植体-基台连接处及其周围的细菌存在非常重要。
锁定锥度设计所提供的密封已被证明在体外对细菌侵入具有密封性。
