Zhai Jing-Jing, Zou Jing, Lu Li-Ying
State Key Laboratory of Oral Diseases, Sichuan University, Chengdu 610041, China.
Hua Xi Kou Qiang Yi Xue Za Zhi. 2009 Dec;27(6):618-21.
To explore a selected-media of Bifidobacterium from oral cavity, to detect the distribution of Bifidobacterium in different sites of children and primarily investigate the relationship between oral Bifidobacterium and early childhood caries.
70 children aged from 3 to 5-year-old were selected, 30 children were caries-free and 40 were severe early childhood caries (S-ECC). Saliva was collected and plaque samples from the 30 healthy subjects were pooled. For S-ECC group, plaques were collected separately from four different sites as follows: Saliva, surfaces of intact enamel, surfaces of white spot-lesions, and deep dentin-lesions. Samples would be grown in the selected-media, and the whole DNA of bacteria was extracted. Polymerase chain reaction was performed with specific primers and the results were analyzed by the electrophoresis.
Bifidobacterium were detected 0 in the caries-free children, while 47.5% in the S-ECC group. There was significant difference between two groups (P < 0.05) and there was no difference between different sites of teeth in S-ECC group (P > 0.05). 27.5% Bifidobacterium were detected in saliva, 27.5% on surfaces of intact enamel, 20.0% on surfaces of white spot-lesions and 22.5% in deep dentin-lesions. 10% Bifidobacterium dentium were detected in saliva, 7.5% on surfaces of intact enamel, 7.5% on surfaces of white spot-lesions and 10.0% in deep dentin-lesions.
One type of modified selected media of Bifidobacterium in oral cavity was explored. Bifidobacterium may be related to the occurrence of the S-ECC and has nothing to do with different sites of teeth in children.
探索口腔双歧杆菌的一种选择培养基，检测双歧杆菌在儿童不同部位的分布，并初步研究口腔双歧杆菌与幼儿早期龋的关系。
选取70名3至5岁儿童，其中30名无龋儿童，40名患有重度幼儿早期龋（S-ECC）。收集唾液，并将30名健康受试者的菌斑样本合并。对于S-ECC组，从以下四个不同部位分别收集菌斑：唾液、完整釉质表面、白斑病变表面和深层牙本质病变。样本将在选择培养基中培养，提取细菌的全基因组DNA。使用特异性引物进行聚合酶链反应，并通过电泳分析结果。
无龋儿童中未检测到双歧杆菌，而S-ECC组中检测到率为47.5%。两组之间存在显著差异（P<0.05），S-ECC组牙齿不同部位之间无差异（P>0.05）。在唾液中检测到27.5%的双歧杆菌，在完整釉质表面检测到27.5%，在白斑病变表面检测到20.0%，在深层牙本质病变中检测到22.5%。在唾液中检测到10%的龋齿双歧杆菌，在完整釉质表面检测到7.5%，在白斑病变表面检测到7.5%，在深层牙本质病变中检测到10.0%。
探索了一种改良的口腔双歧杆菌选择培养基。双歧杆菌可能与S-ECC的发生有关，与儿童牙齿的不同部位无关。
