Department of Biochemical Technology, Institute of Biotechnology and Food Science, Faculty of Chemical and Food Technology, Slovak University of Technology in Bratislava, Slovak Republic.
J Basic Microbiol. 2010 Apr;50(2):135-42. doi: 10.1002/jobm.200900227.
In the course of study of epiphytic microorganisms occurring on the surface of roots of Taxus baccata L. a new strain Streptomyces sp. AC113 was isolated. According to 16S ribosomal DNA-based identification the new strain is 99% identical with Streptomyces flavidofuscus. This strain cultivated in an arginine glycerol medium produced three major metabolites identified as (-)-8-O -methyltetrangomycin (1), 8-O -methyltetrangulol (2) and 8-O -methyl-7-deoxo-7-hydroxytetrangomycin (3). The chemical structures of these angucyclines were elucidated by 1D and 2D NMR as well as by mass spectrometry. Isolated angucycline metabolites showed significant antimicrobial activity against Bacillus cereus and Listeria mocytogenes. Cytotoxic activities of compounds 1, 2 and 3 against four cell lines (B16, HT-29 and non - tumor V79, L929) were evaluated. Compound 3 was the most potent anticancer agents with IC(50) 0.054 microg/ml against cell line B16.
在研究 Taxus baccata L. 根表面附生微生物的过程中，分离到一株新的链霉菌 AC113。根据 16S 核糖体 DNA 的鉴定，该新菌株与 Streptomyces flavidofuscus 的相似度为 99%。该菌株在精氨酸甘油培养基中培养，产生了三种主要代谢产物，分别鉴定为 (-)-8-O-甲基四氢蒽霉素（1）、8-O-甲基四氢蒽醇（2）和 8-O-甲基-7-去氧-7-羟基四氢蒽霉素（3）。这些蒽环类化合物的化学结构通过 1D 和 2D NMR 以及质谱法阐明。分离得到的蒽环类代谢产物对蜡状芽孢杆菌和李斯特菌表现出显著的抗菌活性。对四种细胞系（B16、HT-29 和非肿瘤 V79、L929）进行了化合物 1、2 和 3 的细胞毒性活性评估。化合物 3 是最有效的抗癌剂，对 B16 细胞系的 IC50 为 0.054 微克/毫升。
