School of Biotechnology, University of Jammu, Jammu, J & K, India.
J Basic Microbiol. 2010 Feb;50(1):110-4. doi: 10.1002/jobm.200900295.
Endophytes include all organisms that during a variable period of their life, colonize the living internal tissues of their hosts without causing detectable symptoms. Several fungal endophytes have been isolated from a variety of plant species which have proved themselves as a rich source of secondary metabolites. The reported natural products from endophytes include antibiotics, immunosuppresants, anticancer compounds, antioxidant agents, etc. For the first time Rosa damacaena (rose) has been explored for its endophytes. The rose oil industry is the major identified deligence for its application in perfumery, flavouring, ointments, and pharmaceuticals including various herbal products. During the present investigation fungal endophytes were isolated from Rosa damacaena. A total of fifty four isolates were isolated out of which sixteen isolates were screened for the production of secondary metabolites. GCMS analysis reveals the production of 2-phenylethanol by one of the isolates JUBT 3M which was identified as Aspergillus niger. This is the first report of production of 2-phenylethanol from endophytic A. niger. 2-phenylethanol is an important constituent of rose oil constituting about 4.06% of rose oil. Presence of 2-phenylethanol indicates that the endophyte of rose may duplicate the biosynthesis of phenyl propanoids by rose plant. Besides this, the other commercial applications of phenylethanol include its use in antiseptics, disinfectants, anti-microbials and preservative in pharmaceuticals.
内生菌包括所有在其生命的某个可变时期内，定植于其宿主的活内部组织而不引起可察觉症状的生物体。已经从各种植物物种中分离出几种真菌内生菌，这些内生菌被证明是次生代谢产物的丰富来源。从内生菌中报道的天然产物包括抗生素、免疫抑制剂、抗癌化合物、抗氧化剂等。这是第一次对大马士革玫瑰（玫瑰）的内生菌进行探索。玫瑰油产业是其在香水、调味、软膏和制药（包括各种草药产品）中应用的主要识别用途。在目前的调查中，从大马士革玫瑰中分离出了真菌内生菌。总共分离出 54 株，其中 16 株被筛选用于次生代谢产物的产生。GCMS 分析显示，一种名为 Aspergillus niger 的内生菌 JUBT 3M 产生了 2-苯乙醇。这是首次从内生 Aspergillus niger 中生产 2-苯乙醇的报道。2-苯乙醇是玫瑰油的重要成分，占玫瑰油的 4.06%左右。2-苯乙醇的存在表明，玫瑰的内生菌可能通过玫瑰植物复制苯丙烷类生物合成。除此之外，苯乙醇的其他商业应用包括在制药中用作防腐剂、消毒剂、抗菌剂和防腐剂。
