Department of Chemistry and Environmental Sciences, New Jersey Institute of Technology, 151 Tiernan Hall, University Heights, Newark, New Jersey 07102, USA.
Bioconjug Chem. 2010 Feb 17;21(2):319-27. doi: 10.1021/bc900403n.
Novel amino-reactive derivatives of lanthanide-based luminescent labels of enhanced brightness and metal retention were synthesized and used for the detection of cDNA oligonucleotides by molecular beacons. Time-resolved acquisition of the luminescent signal that occurs upon hybridization of the probe to the target enabled the avoidance of short-lived background fluorescence, markedly enhancing the sensitivity of detection, which was less than 1 pM. This value is about 50 to 100 times more sensitive than the level achieved with conventional fluorescence-based molecular beacons, and is 10 to 60 times more sensitive than previously reported for other lanthanide-based hybridization probes. These novel luminescent labels should significantly enhance the sensitivity of all type of nucleic acid hybridization probes, and could dramatically improve the detection limit of other biopolymers and small compounds that are used in a variety of biological applications.
新型氨基酸反应性镧系元素发光标记物具有增强的亮度和金属保留率，被合成并用于通过分子信标检测 cDNA 寡核苷酸。通过对探针与靶标杂交时发生的发光信号进行时间分辨采集，可以避免短暂的背景荧光，显著提高检测灵敏度，检测限小于 1 pM。与传统基于荧光的分子信标相比，该值的灵敏度提高了约 50 至 100 倍，与之前报道的其他基于镧系元素的杂交探针相比，灵敏度提高了 10 至 60 倍。这些新型发光标记物应该显著提高所有类型的核酸杂交探针的灵敏度，并可以极大地提高用于各种生物应用的其他生物聚合物和小分子的检测限。
