Centro Nacional de Ciencia y Tecnología de Alimentos, Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, Codigo Postal 11501-2060, San José, Costa Rica.
J Agric Food Chem. 2010 Feb 10;58(3):1455-61. doi: 10.1021/jf903467x.
The novel method developed for screening cellular antioxidant activity relies on differences in light-scattering properties (turbidity) between intact and lysed human erythrocytes. AAPH, a peroxyl radical generator, was used to enhance lipid peroxidation. The consequent hemolysis triggered a loss of the light-scattering ability in the lysed erythrocytes. When an antioxidant was added, the area under the absorbance decay curve (AUC) was linearly proportional to the concentration of antioxidant compound. This erythrocyte cellular antioxidant activity (ERYCA) method was found to be relatively fast, sensitive, accurate, and repeatable, even when using erythrocytes from different donors and for different storage times. The method was used to assess the antioxidant capacity of pure phenolic compounds, fruit juices, stimulant beverages, and blood plasma and compared with ORAC values. The values resulting from the two methods did not correlate as the mechanisms involved were different.
本研究开发的新型细胞抗氧化活性筛选方法依赖于完整和裂解人红细胞之间的光散射特性（浊度）差异。AAPH（过氧自由基生成剂）用于增强脂质过氧化。随后的溶血触发了裂解红细胞光散射能力的丧失。当添加抗氧化剂时，吸光度衰减曲线下面积（AUC）与抗氧化剂化合物的浓度呈线性比例关系。发现该红细胞细胞抗氧化活性（ERYCA）方法相对快速、敏感、准确且可重复，即使使用来自不同供体和不同储存时间的红细胞也是如此。该方法用于评估纯酚类化合物、果汁、兴奋剂饮料和血浆的抗氧化能力，并与 ORAC 值进行比较。两种方法得到的结果没有相关性，因为所涉及的机制不同。
