The goal of the current study is to achieve plant-mediated synthesis of iron oxide nanoparticles (FeO NPs). The plant extract of was used as a reducing and stabilizing agent for the synthesis of FeO NPs. Different techniques such as energy-dispersive X-ray analysis (EDX), X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FT-IR), and UV-visible spectroscopy (UV-vis) were used to characterize the synthesis of FeO NPs. UV-visible spectroscopy verified the synthesis of FeO NPs using a surface plasmon resonance peak at a wavelength of 370 nm. SEM analysis specifies the spherical morphology of the synthesized nanoparticles with a size range between 30 and 70 nm. The reducing and capping materials of FeO NPs were revealed by FT-IR analysis based on functional group identification. The plant extract contained essential functional groups, such as C-H, C-O, N-H, -CH, and -OH, that facilitate the green synthesis of FeO NPs. The EDX analysis detected the atomic percentage with the elemental composition of FeO NPs, while the XRD pattern demonstrated the crystallinity of FeO NPs. Furthermore, the synthesized FeO NPs showed potential antiglycation activity under conditions, which was confirmed by the efficient zone of inhibition on glycation of bovine serum albumin/glucose (BSA-glucose) in the order <100 < 500 < 1000 μg/mL, which revealed that FeO NPs showed significant antiglycation activity. Additionally, the cytotoxic activity against brain glioblastoma cells was assessed using the MTT assay, which exhibited diminished cytotoxic activity at concentrations lower than 300 μg/mL. Thus, we assumed that the resulting FeO NPs are a good option for use in drug delivery and cancer treatments.