从大鼠垂体中分离和鉴定阿片肽：β-内啡肽。
Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.
作者信息
Rubinstein M, Stein S, Udenfriend S
出版信息
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4969-72. doi: 10.1073/pnas.74.11.4969.
Abstract
beta-Endorphin was isolated from 200 rat pituitaries by means of high-performance column chromatography, using sensitive fluorometric methods and a radioreceptor assay for opioid activity. The beta-endorphin was characterized as to molecular weight, amino acid composition, and mapping of tryptic peptides by a new microtechnique. It was found that rat beta-endorphin is identical to camel and sheep beta-endorphin. Furthermore, alpha-endorphin, Met-enkephalin, a nonpeptide morphine-like compound (MLC), and an additional unidentified opioid compound were detected in the extract of rat pituitary.
摘要
通过高效柱色谱法，利用灵敏的荧光测定法和阿片样物质活性的放射受体分析法，从200个大鼠垂体中分离出β-内啡肽。采用一种新的微技术对β-内啡肽的分子量、氨基酸组成和胰蛋白酶肽图谱进行了表征。发现大鼠β-内啡肽与骆驼和绵羊的β-内啡肽相同。此外，在大鼠垂体提取物中还检测到了α-内啡肽、甲硫氨酸脑啡肽、一种非肽类吗啡样化合物（MLC）以及另一种未鉴定的阿片样化合物。
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