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从大鼠垂体中分离和鉴定阿片肽：β-内啡肽。

Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.

作者信息

Rubinstein M, Stein S, Udenfriend S

出版信息

Proc Natl Acad Sci U S A. 1977 Nov;74(11):4969-72. doi: 10.1073/pnas.74.11.4969.

DOI:10.1073/pnas.74.11.4969
PMID:270731
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432079/
Abstract

beta-Endorphin was isolated from 200 rat pituitaries by means of high-performance column chromatography, using sensitive fluorometric methods and a radioreceptor assay for opioid activity. The beta-endorphin was characterized as to molecular weight, amino acid composition, and mapping of tryptic peptides by a new microtechnique. It was found that rat beta-endorphin is identical to camel and sheep beta-endorphin. Furthermore, alpha-endorphin, Met-enkephalin, a nonpeptide morphine-like compound (MLC), and an additional unidentified opioid compound were detected in the extract of rat pituitary.

摘要

通过高效柱色谱法，利用灵敏的荧光测定法和阿片样物质活性的放射受体分析法，从200个大鼠垂体中分离出β-内啡肽。采用一种新的微技术对β-内啡肽的分子量、氨基酸组成和胰蛋白酶肽图谱进行了表征。发现大鼠β-内啡肽与骆驼和绵羊的β-内啡肽相同。此外，在大鼠垂体提取物中还检测到了α-内啡肽、甲硫氨酸脑啡肽、一种非肽类吗啡样化合物（MLC）以及另一种未鉴定的阿片样化合物。

相似文献

1
Isolation and characterization of the opioid peptides from rat pituitary: beta-endorphin.从大鼠垂体中分离和鉴定阿片肽：β-内啡肽。
Proc Natl Acad Sci U S A. 1977 Nov;74(11):4969-72. doi: 10.1073/pnas.74.11.4969.
2
Beta-endorphin-related peptides in the human pituitary. Isolation and characterization of major immunoreactive peptides, including the formerly unrecognized peptide beta-endorphin 1-18.人垂体中与β-内啡肽相关的肽。主要免疫反应性肽的分离与鉴定，包括之前未被识别的肽β-内啡肽1-18。
Acta Physiol Scand Suppl. 1984;531:1-84.
3
beta-Endorphin: isolation, amino acid sequence and synthesis of the hormone from horse pituitary glands.β-内啡肽：从马垂体中分离、氨基酸序列及该激素的合成。
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Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.从大鼠垂体中分离和鉴定阿片样肽：β-促脂素。
Proc Natl Acad Sci U S A. 1977 Jul;74(7):3052-5. doi: 10.1073/pnas.74.7.3052.
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alpha-N-acetyl-beta-endorphin1-26 from the neurointermediary lobe of the rat pituitary: isolation, purification, and characterization by high-performance liquid chromatography.大鼠垂体神经中间叶的α-N-乙酰-β-内啡肽1-26：通过高效液相色谱法进行分离、纯化及特性鉴定
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Isolation and properties of beta-endorphin-(1-27), N alpha-acetyl-beta-endorphin, corticotropin, gamma-lipotropin and neurophysin from equine pituitary glands.从马垂体中分离出β-内啡肽-（1-27）、Nα-乙酰-β-内啡肽、促肾上腺皮质激素、γ-促脂素和神经垂体素及其性质
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Identification of N alpha-acetyl-alpha-endorphin and N alpha-acetyl-gamma-endorphin isolated from the neurointermediate lobe of the rat pituitary gland.从大鼠垂体神经中间叶分离出的Nα-乙酰-α-内啡肽和Nα-乙酰-γ-内啡肽的鉴定。
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Isolation and characterization of alpha-endorphin and gamma-endorphin from single human pituitary glands.从单个人类垂体中分离并鉴定α-内啡肽和γ-内啡肽。
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Characterization of pro-opiocortin, a precursor to opioid peptides and corticotropin.阿片肽和促肾上腺皮质激素的前体——阿片促皮质素原的特性分析
Proc Natl Acad Sci U S A. 1978 Feb;75(2):669-71. doi: 10.1073/pnas.75.2.669.

引用本文的文献

1
Isolation, characterization and opiate activity of beta-endorphin from the pituitary gland of the ostrich, Struthio camelus.鸵鸟（鸵鸟属驼鸟）垂体中β-内啡肽的分离、特性鉴定及阿片样活性
Biochem J. 1980 Apr 1;187(1):245-8. doi: 10.1042/bj1870245.
2
Distribution of beta-endorphin-related peptides in rat pituitary and brain.β-内啡肽相关肽在大鼠垂体和脑中的分布。
Biochem J. 1982 Mar 15;202(3):561-71. doi: 10.1042/bj2020561.
3
Prediction of peptide retention times in high-pressure liquid chromatography on the basis of amino acid composition.基于氨基酸组成预测高压液相色谱中肽的保留时间。
Proc Natl Acad Sci U S A. 1980 Mar;77(3):1632-6. doi: 10.1073/pnas.77.3.1632.
4
Vasopressin stimulates release of beta-lipotropin and beta-endorphin in conscious rats as measured by radioimmunoassay of unextracted plasma.通过对未提取血浆进行放射免疫测定可知，血管加压素可刺激清醒大鼠体内β-促脂素和β-内啡肽的释放。
Naunyn Schmiedebergs Arch Pharmacol. 1981 Feb;316(1):59-63. doi: 10.1007/BF00507229.
5
Chemistry and biosynthesis of pro-opiomelanocortin. ACTH, MSH's, endorphins and their related peptides.促肾上腺皮质激素原的化学与生物合成。促肾上腺皮质激素、促黑素、内啡肽及其相关肽。
Mol Cell Biochem. 1981 Jan 28;34(2):101-27. doi: 10.1007/BF02354864.
6
Isolation and characterization of beta-endorphin-like peptides from bovine brains.从牛脑中分离并鉴定β-内啡肽样肽
Proc Natl Acad Sci U S A. 1980 Jan;77(1):230-3. doi: 10.1073/pnas.77.1.230.
7
Distribution of active and inactive forms of endorphins in rat pituitary and brain.内啡肽的活性和非活性形式在大鼠垂体和大脑中的分布。
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5972-6. doi: 10.1073/pnas.76.11.5972.
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Pituitary somatotrophs contain [Met]enkephalin-like immunoreactivity.垂体生长激素细胞含有[甲硫氨酸]脑啡肽样免疫反应性物质。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):6134-8. doi: 10.1073/pnas.75.12.6134.
9
Human leukocyte interferon: production, purification to homogeneity, and initial characterization.人白细胞干扰素：生产、纯化至均一性及初步特性鉴定
Proc Natl Acad Sci U S A. 1979 Feb;76(2):640-4. doi: 10.1073/pnas.76.2.640.
10
High molecular weight opioid-containing proteins in striatum.纹状体中含高分子量阿片样物质的蛋白质
Proc Natl Acad Sci U S A. 1978 Aug;75(8):4021-3. doi: 10.1073/pnas.75.8.4021.

本文引用的文献

1
Amino acid analysis with fluorescamine at the picomole level.皮摩尔水平的荧光胺氨基酸分析。
Arch Biochem Biophys. 1973 Mar;155(1):202-12. doi: 10.1016/s0003-9861(73)80022-0.
2
Automatic Monitoring of primary amines in preparative column effluents with fluorescamine.使用荧光胺自动监测制备柱流出物中的伯胺。
Anal Biochem. 1975 Aug;67(2):438-45. doi: 10.1016/0003-2697(75)90316-4.
3
Antibodies as a means of isolating and characterizing biologically active substances: presence of a non-peptide, morphine-like compound in the central nervous system.抗体作为分离和鉴定生物活性物质的手段：中枢神经系统中存在一种非肽类、吗啡样化合物。
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2132-6. doi: 10.1073/pnas.73.6.2132.
4
Isolation and structure of an untriakontapeptide with opiate activity from camel pituitary glands.从骆驼脑垂体中分离出具有阿片样活性的三十一肽并解析其结构。
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1145-8. doi: 10.1073/pnas.73.4.1145.
5
Homology between a prolyl hydroxylase subunit and a tissue protein that crossreacts immunologically with the enzyme.脯氨酰羟化酶亚基与一种可与该酶发生免疫交叉反应的组织蛋白之间的同源性。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4420-4. doi: 10.1073/pnas.74.10.4420.
6
Isolation and characterization of the opioid peptides from rat pituitary: beta-lipotropin.从大鼠垂体中分离和鉴定阿片样肽：β-促脂素。
Proc Natl Acad Sci U S A. 1977 Jul;74(7):3052-5. doi: 10.1073/pnas.74.7.3052.