Hu Hai-Yan, Dou Xing-Ru, Jiang Zong-Lin, Tang Jian-Hua, Xie Lian, Xie Hong-Ping
College of Chemistry and Chemical Engineering, China West Normal University, Nanchong 637002, P.R. China.
College of Pharmaceutical Science, Soochow University, Suzhou 215123, P.R. China.
J Pharm Anal. 2012 Aug;2(4):293-297. doi: 10.1016/j.jpha.2012.02.003. Epub 2012 Feb 21.
The nanocomposites of poly-diallyldimethylammonium chloride (PDADMAC) and CdTe quantum dots (QDs) (i.e. QDs-PDADMAC nanocomposites) have been prepared based on electrostatic interaction and their fluorescence stability in aqueous solution has been investigated. MTT method (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide method) was used to study their cytotoxicity and A549 lung cancer cell as a model cell was also used to evaluate their cellular imaging. It was shown that the fluorescence stability of QDs-PDADMAC nanocomposites was much better than that of bare QDs both in aqueous solution and cell. Meanwhile, QDs-PDADMAC nanocomposites display very low cytotoxicity in the low concentrations and better staining ability compared with QDs. QDs-PDADMAC nanocomposites will have great advantage on the cell analysis detection and imaging.
基于静电相互作用制备了聚二烯丙基二甲基氯化铵（PDADMAC）与碲化镉量子点（QDs）的纳米复合材料（即QDs-PDADMAC纳米复合材料），并研究了其在水溶液中的荧光稳定性。采用MTT法（3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐法）研究其细胞毒性，还以A549肺癌细胞作为模型细胞评估其细胞成像。结果表明，QDs-PDADMAC纳米复合材料在水溶液和细胞中的荧光稳定性均远优于裸量子点。同时，QDs-PDADMAC纳米复合材料在低浓度下显示出极低的细胞毒性，且与量子点相比具有更好的染色能力。QDs-PDADMAC纳米复合材料在细胞分析检测和成像方面将具有很大优势。
