Department of Nutrition, School of Nutrition, Chung Shan Medical University, Chung Shan Medical University Hospital, No 110, Section 1, Jianguo North Road, Taichung 40201, Taiwan.
J Agric Food Chem. 2010 Feb 24;58(4):2150-6. doi: 10.1021/jf903557c.
Orthosiphon aristatus (Blume) Miq., which can be used as a food ingredient, is grown throughout Southeast Asia and Australia. O. aristatus is frequently used for the treatment of renal inflammation, kidney stones and dysuria. The focus of the current work was to study the antioxidant and anti-inflammatory effects of methanol, ethanol and water extracts from O. aristatus (abbreviated as MEOA, EEOA and WEOA, respectively). The evaluation of antioxidant activity was determined by total phenolics, Trolox equivalent antioxidant capacity (TEAC), oxygen-radical absorbance capacity (ORAC) and cellular antioxidant activity (CAA) assays. These assays demonstrated a relatively high antioxidant activity for MEOA and EEOA. These results revealed that EEOA had the most prominent inhibitory effect on lipopolysaccharide (LPS)-stimulated nitric oxide (NO), prostaglandin E(2) (PGE(2)) and intracellular reactive oxygen species (ROS) production in RAW 264.7 cells. A high performance liquid chromatography profile indicated that MEOA and EEOA contained both ursolic acid and oleanolic acid. Moreover, ursolic acid significantly reduced NO production in LPS-stimulated RAW 264.7 cells. Both EEOA and ursolic acid inhibited LPS-stimulated protein and mRNA expression of both inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in these cells. These results demonstrate that EEOA and its bioactive compound, ursolic acid, suppress LPS-induced NO and PGE(2) production by inhibiting ROS generation, along with reducing expression of iNOS and COX-2 in RAW 264.7 cells.
越南肾茶（Blume）Miq.，可用作食品成分，在东南亚和澳大利亚均有种植。越南肾茶常用于治疗肾炎、肾结石和尿痛。本研究的重点是研究越南肾茶甲醇、乙醇和水提取物（分别缩写为 MEOA、EEOA 和 WEOA）的抗氧化和抗炎作用。抗氧化活性的评估通过总酚、Trolox 当量抗氧化能力（TEAC）、氧自由基吸收能力（ORAC）和细胞抗氧化活性（CAA）测定来确定。这些测定结果表明 MEOA 和 EEOA 具有相对较高的抗氧化活性。这些结果表明，EEOA 对 LPS 刺激的 RAW 264.7 细胞中一氧化氮（NO）、前列腺素 E2（PGE2）和细胞内活性氧（ROS）的产生具有最显著的抑制作用。高效液相色谱图表明，MEOA 和 EEOA 均含有熊果酸和齐墩果酸。此外，熊果酸显著降低了 LPS 刺激的 RAW 264.7 细胞中 NO 的产生。EEOA 和熊果酸均抑制 LPS 刺激的诱导型一氧化氮合酶（iNOS）和环氧化酶-2（COX-2）在这些细胞中的蛋白和 mRNA 表达。这些结果表明，EEOA 及其生物活性化合物熊果酸通过抑制 ROS 生成，降低 RAW 264.7 细胞中 iNOS 和 COX-2 的表达，抑制 LPS 诱导的 NO 和 PGE2 的产生。
