Department of Biotechnology, Polytechnic University of Valencia, Valencia, Spain.
Microbiol Res. 2010 Oct 20;165(8):657-64. doi: 10.1016/j.micres.2009.11.012. Epub 2010 Jan 27.
Vibrio vulnificus is an opportunistic human pathogen that may cause gastroenteritis, severe necrotizing soft-tissue infections and primary septicaemia, with a high lethality rate. Illness is associated to ingestion of seafood or to the exposure of contaminated water. The aim of this work was to determine the occurrence of V. vulnificus in water and seafood samples from a coastal area near the Mediterranean (Valencia, Spain). A TaqMan probe-based real-time PCR assay was optimised and applied to 22 sea water, 42 raw sewage and 40 seafood samples. Results were compared with those obtained for culture isolation. The detection level of the PCR assay was 10 CFU g⁻¹ in inoculated samples. Seven seawater, four shellfish and six wastewater samples were positive by real time PCR. V. vulnificus was isolated from two oyster, three sea water and two wastewater samples. All the strains were obtained after 20 h enrichment, except for wastewater strains, which were isolated directly from the sample. To our knowledge, this is the first report on the isolation of V. vulnificus from sewage in Spain. Our results about the presence of V. vulnificus in food and environmental samples are strong enough to consider that the organism may represent a human health hazard in our geographical area.
创伤弧菌是一种机会性人类病原体，可导致肠胃炎、严重坏死性软组织感染和原发性败血症，致死率很高。这种疾病与食用海鲜或接触受污染的水有关。本工作旨在确定在地中海附近（西班牙瓦伦西亚）沿海地区的水和海鲜样本中创伤弧菌的发生情况。优化了一种基于 TaqMan 探针的实时 PCR 检测方法，并应用于 22 份海水、42 份未经处理的污水和 40 份海鲜样本。将结果与培养分离的结果进行了比较。PCR 检测方法的检测限为接种样本中的 10 CFU g⁻¹。实时 PCR 检测结果显示，7 份海水、4 份贝类和 6 份污水呈阳性。从 2 份牡蛎、3 份海水和 2 份污水样本中分离到创伤弧菌。所有菌株均在 20 小时的富集后获得，污水菌株除外，它们直接从样本中分离得到。据我们所知，这是西班牙首次从污水中分离出创伤弧菌的报道。我们关于食品和环境样本中存在创伤弧菌的结果足以表明，在我们的地理区域，该生物体可能对人类健康构成威胁。
