Department of Medicine, Boston University, Massachusetts, USA.
J Gerontol A Biol Sci Med Sci. 2010 Mar;65(3):242-51. doi: 10.1093/gerona/glp213. Epub 2010 Jan 27.
Fat distribution changes with aging. Inherent changes in fat cell progenitors may contribute because fat cells turn over throughout life. To define mechanisms, gene expression was profiled in preadipocytes cultured from epididymal and perirenal depots of young and old rats. 8.4% of probe sets differed significantly between depots, particularly developmental genes. Only 0.02% differed with aging, despite using less stringent criteria than for comparing depots. Twenty-five genes selected based on fold change with aging were analyzed in preadipocytes from additional young, middle-aged, and old animals by polymerase chain reaction. Thirteen changed significantly with aging, 13 among depots, and 9 with both. Genes involved in inflammation, stress, and differentiation changed with aging, as occurs in fat tissue. Age-related changes were greater in perirenal than epididymal preadipocytes, consistent with larger declines in replication and adipogenesis in perirenal preadipocytes. Thus, age-related changes in preadipocyte gene expression differ among depots, potentially contributing to fat redistribution and dysfunction.
脂肪分布随年龄而变化。脂肪细胞祖细胞的固有变化可能是造成这种变化的原因，因为脂肪细胞在整个生命周期中都会更新。为了明确机制，我们对从小鼠附睾和肾周脂肪组织培养的前体脂肪细胞中的基因表达进行了分析。8.4%的探针在不同部位之间差异显著，特别是发育基因。尽管与比较不同部位相比，使用的标准不那么严格，但只有 0.02%的基因随年龄而变化。根据与衰老相关的倍数变化，从额外的年轻、中年和老年动物的前体脂肪细胞中选择了 25 个基因进行聚合酶链反应分析。其中 13 个与衰老相关，13 个与部位相关，9 个与两者都相关。与炎症、应激和分化相关的基因随衰老而变化，就像在脂肪组织中一样。肾周前体脂肪细胞的衰老相关变化大于附睾前体脂肪细胞，这与肾周前体脂肪细胞的复制和脂肪生成能力下降更大有关。因此，前体脂肪细胞基因表达的衰老相关变化在不同部位之间存在差异，这可能是导致脂肪重新分布和功能障碍的原因之一。
