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新型人乙型肝炎核心抗体双抗原夹心免疫测定法

Novel double-antigen sandwich immunoassay for human hepatitis B core antibody.

作者信息

Li An, Yuan Quan, Huang Zhiyin, Fan Jian, Guo Ruyi, Lou Bin, Zheng Qin, Ge Shengxiang, Chen Yixin, Su Zhijun, Yeo Anthony E T, Chen Yu, Zhang Jun, Xia Ningshao

机构信息

National Institute of Diagnostics and Vaccine Development in Infectious Diseases, School of Life Science, Xiamen University, Xiamen, Fujian Province 361005, People's Republic of China.

出版信息

Clin Vaccine Immunol. 2010 Mar;17(3):464-9. doi: 10.1128/CVI.00457-09. Epub 2010 Jan 27.

DOI:10.1128/CVI.00457-09
PMID:20107008
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2837963/
Abstract

A novel diagnostic immunoassay testing procedure for hepatitis B virus core antibody (anti-HBc) using homogeneous purified full-length hepatitis B virus core antigen (HBcAg) capsids obtained from Escherichia coli was compared with Abbott Architect anti-HBc chemiluminescent microparticle immunoassay (CMIA; indirect method) against a library of specimens. A monoclonal anti-HBc neutralization confirmatory assay was then used to determine the degree of discordance between specimens. The new assay was found to be superior in both sensitivity and specificity.

摘要

使用从大肠杆菌中获得的均一纯化全长乙型肝炎病毒核心抗原（HBcAg）衣壳，对一种用于检测乙型肝炎病毒核心抗体（抗-HBc）的新型诊断免疫测定测试程序，与雅培Architect抗-HBc化学发光微粒子免疫测定法（CMIA；间接法）针对一组标本库进行了比较。然后使用单克隆抗-HBc中和确证试验来确定标本之间的不一致程度。发现新测定法在敏感性和特异性方面均更具优势。

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Detection of antibodies to hepatitis B core antigen using the Abbott ARCHITECT anti-HBc assay: analysis of borderline reactive sera.使用雅培ARCHITECT抗-HBc检测法检测乙型肝炎核心抗原抗体：临界反应性血清分析
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Can the serological status of anti-HBc alone be considered a sentinel marker for detection of occult HBV infection?仅抗-HBc的血清学状态能否被视为检测隐匿性HBV感染的哨兵标志物？
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Serological pattern "anti-HBc alone": characterization of 552 individuals and clinical significance.血清学模式“单独抗-HBc”：552例个体的特征及临床意义
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