Mischak Harald, Julian Bruce A, Novak Jan
Mosaiques Diagnostics & Therapeutics, Hannover, Germany.
Proteomics Clin Appl. 2007 Jul 10;1(8):792. doi: 10.1002/prca.200700043.
All organisms contain thousands of proteins and peptides in their body fluids. A deeper insight into the functional relevance of these polypeptides under different physiological and pathophysiological conditions and the discovery of specific peptide biomarkers would greatly enhance diagnosis and therapy of specific diseases. The low-molecular-weight proteome, also termed peptidome, provides a rich source of information. Due to its unique features, the technical challenges differ somewhat from those in "common" proteomics. In this manuscript, we focus on the low-molecular-weight urinary proteome. We review the methodological aspects of sample collection, preparation, analysis, and subsequent data evaluation. In the second part of this review, we summarize the recent progress in the definition and identification of clinically relevant polypeptide markers.
所有生物体的体液中都含有数千种蛋白质和肽。深入了解这些多肽在不同生理和病理生理条件下的功能相关性，并发现特定的肽生物标志物，将极大地提高特定疾病的诊断和治疗水平。低分子量蛋白质组，也称为肽组，提供了丰富的信息来源。由于其独特的特性，技术挑战与“常规”蛋白质组学有所不同。在本手稿中，我们聚焦于低分子量尿液蛋白质组。我们回顾了样本采集、制备、分析以及后续数据评估的方法学方面。在本综述的第二部分，我们总结了在临床相关多肽标志物的定义和鉴定方面的最新进展。
