Department of Anesthesiology, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou 510080, PR China.
Lipids Health Dis. 2010 Jan 28;9:9. doi: 10.1186/1476-511X-9-9.
Polysaccharide from fuzi (FPS), a Chinese herbal medicine extract, has been demonstrated to exert lipid lowering affects. In this study we examined potential mechanisms underlying this affect, specifically alterations in expression of the LDL-receptor (LDL-R), 3-hydroxy-3-methyl glutaryl (HMG)-CoA reductase and cytochrome P450 7alpha-1 (CYP7alpha-1), using a rat model of hypercholesterolemia.
Male rats were fed either a normal or high cholesterol (HC) diet for two-weeks. Half of the rats on the HC diet were orally gavaged with FPS (224 mg/kg, 448 mg/kg or 896 mg/kg diet) daily. Serum lipid levels were quantified at end of the study period as were liver levels of LDL-R protein and mRNA expression of CYP7alpha-1 and HMG-CoA. Serum cholesterol and LDL-C concentrations were significantly elevated from control in HC rats, but not in those treated with FPS (P < 0.05). LDL-R expression was significantly decreased in the HC group compared to control (P < 0.05), but significantly increased in the FPS group (P < 0.05). HMG-CoA mRNA levels were significantly increased in the HC group compared both other groups (P < 0.05), while CYP7alpha-1 expression was significantly higher in the FPS group compared to both other groups (P < 0.05).
These findings suggest that the cholesterol lowering effect of FPS in hypercholesteremic rats is caused at least in part by increased hepatic LDL-R and CYP7alpha-1 expression and decreased HMG-CoA expression. Further study is needed to determine precisely where and how FPS exerts these effects. FPS offers potential as a therapeutic agent for the treatment of hypercholesterolemia.
从中药附子中提取的多糖（FPS）已被证明具有降低血脂的作用。在这项研究中，我们使用高胆固醇血症大鼠模型，研究了这种作用的潜在机制，特别是 LDL 受体（LDL-R）、3-羟-3-甲基戊二酰辅酶 A（HMG-CoA）还原酶和细胞色素 P450 7α-1（CYP7α-1）表达的变化。
雄性大鼠连续两周分别喂食正常或高胆固醇（HC）饮食。一半的 HC 饮食大鼠每天口服给予 FPS（224mg/kg、448mg/kg 或 896mg/kg 饮食）。研究结束时，定量检测血清脂质水平以及肝脏 LDL-R 蛋白水平和 CYP7α-1 和 HMG-CoA 的 mRNA 表达。与对照组相比，HC 组大鼠血清胆固醇和 LDL-C 浓度显著升高（P < 0.05），但用 FPS 治疗的大鼠则不然（P < 0.05）。与对照组相比，HC 组 LDL-R 表达显著降低（P < 0.05），但 FPS 组显著增加（P < 0.05）。与其他两组相比，HC 组 HMG-CoA mRNA 水平显著升高（P < 0.05），而 FPS 组 CYP7α-1 表达显著高于其他两组（P < 0.05）。
这些发现表明，FPS 在高胆固醇血症大鼠中的降胆固醇作用至少部分是由于肝 LDL-R 和 CYP7α-1 表达增加和 HMG-CoA 表达减少所致。需要进一步研究以确定 FPS 如何以及在何处发挥这些作用。FPS 有望成为治疗高胆固醇血症的治疗剂。
