Department of Biological Science, Florida State University, Tallahassee, 32306-4295, USA.
Brief Funct Genomics. 2010 Mar;9(2):95-102. doi: 10.1093/bfgp/elp058. Epub 2010 Jan 27.
Plant cytogenetics has continued to flourish and make essential contributions to genomics projects by delineating marker order, defining contig gaps and revealing genome rearrangements. Here we review the field of plant cytogenetics from its conception through the eras of molecular biology and genomics. Significant advances in chromosome preparation, such as extended fiber-FISH, have greatly increased the axial resolution limits, while imaging and signal amplification technologies have improved our ability to detect small gene-sized probes. Combinations of traditional FISH technologies with chromatin immunocytochemistry serve to broaden the ability of plant cytogenetics to shed light on genome structure and organization. These advances are described, together with selected examples that illustrate the power of plant cytogenetics in guiding genome projects.
植物细胞遗传学通过标记顺序的描述、重叠群缺口的定义和基因组重排的揭示，继续为基因组学项目做出重要贡献。在这里，我们回顾了从概念到分子生物学和基因组学时代的植物细胞遗传学领域。染色体制备方面的重大进展，如延伸纤维-FISH，极大地提高了轴向分辨率的极限，而成像和信号放大技术提高了我们检测小基因大小探针的能力。传统 FISH 技术与染色质免疫细胞化学的结合，扩大了植物细胞遗传学揭示基因组结构和组织的能力。本文描述了这些进展，并列举了一些例子来说明植物细胞遗传学在指导基因组项目方面的强大功能。
