相思豆碱对克氏锥虫的抗增殖作用及超微结构改变。
Antiproliferative effect and ultrastructural alterations induced by psilostachyin on Trypanosoma cruzi.
机构信息
Cátedra de Farmacognosia, Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, IQUIMEFA (UBA-CONICET), Junín 956 (1113), CABA, Argentina.
出版信息
Molecules. 2010 Jan 25;15(1):545-53. doi: 10.3390/molecules15010545.
Abstract
The effect of psilostachyin, a natural sesquiterpene lactone, on the growth and viability of cultured epimastigotes of Trypanosoma cruzi (Tulahuen) is reported. The antiproliferative effect was evaluated by counting the parasites in a Neubauer chamber and measuring their viability by using the dye exclusion technique. The effect on parasite growth was irreversible at concentrations higher than 1.0 microg/mL and the addition of glutathione only partially blocked the effect of the compound. Moreover, we have studied the effects of this natural compound on parasite ultrastructure by transmission electron microscopy. Interestingly, psilostachyin induced ultrastructural alterations on the parasites at a concentration of 0.5 microg/mL, with important mitochondrial swelling and deformity of the kinetoplast.
摘要
本文报道了天然倍半萜内酯 psilostachyin 对培养的 Trypanosoma cruzi（Tulahuen）epimastigotes 的生长和活力的影响。通过 Neubauer 室计数寄生虫并使用染料排除技术测量其活力来评估抗增殖作用。在浓度高于 1.0μg/mL 时，这种增殖抑制作用是不可逆的，添加谷胱甘肽只能部分阻断该化合物的作用。此外，我们还通过透射电子显微镜研究了该天然化合物对寄生虫超微结构的影响。有趣的是，psilostachyin 在 0.5μg/mL 的浓度下诱导寄生虫出现超微结构改变，线粒体明显肿胀，动基体变形。
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