Wei Qiang, Dai Yi, Wu Yan, Zhang Wen-Jing
Pharmacology College, Anhui Xinhua University, Hefei 230088, China.
Zhong Yao Cai. 2011 Aug;34(8):1285-8.
To study the technology of enriching total flavonoids from Folium Chrysanthemi with polyamide and macroporous resin.
By using the content of flavonoids as index, firstly absorbed with polyamide and removed impurity with purified water. Then added polyamide to macroporous resin column, eluted with ethanol and determined the flavonoids content.
Total flavonoids could be effectively purified by the polyamide and macroporous resin. Best technological conditions were as follows: resin of type X-5 was better, and elution agent was 70% ethanol, the ratio of extract amount (mL) and polyamide quantity (g) was 1. 5: 1, ratio of resin (g) - extract (mL) was 4: 3, ratio of diameter and height was 1: 20, the volume of collected eluent was 2BV. The purity of total flavonoids reached to 87.48%.
The technology is stable and can be viewed as a way to study and develop the resources of Folium Chrysanthemi.
研究用聚酰胺和大孔树脂从野菊花中富集总黄酮的工艺。
以黄酮类化合物含量为指标，先用聚酰胺吸附，再用纯化水去除杂质。然后将聚酰胺加入大孔树脂柱，用乙醇洗脱并测定黄酮类化合物含量。
聚酰胺和大孔树脂可有效纯化总黄酮。最佳工艺条件如下：X-5型树脂较好，洗脱剂为70%乙醇，提取物量（mL）与聚酰胺量（g）之比为1.5:1，树脂（g）-提取物（mL）之比为4:3，直径与高度之比为1:20，收集洗脱液体积为2BV。总黄酮纯度达到87.48%。
该工艺稳定，可作为研究和开发野菊花资源的一种方法。
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