National Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.
Plant Signal Behav. 2010 Apr;5(4):453-7. doi: 10.4161/psb.5.4.10875. Epub 2010 Apr 8.
Ethylene is the first identified gaseous hormone regulating many aspects of plant growth and development. ACC and ethephon are two widely used chemicals replacing ethylene treatment when ethylene is not available. However, the amount of ethylene converted by ACC and ethephon is not controllable, leaving it questionable whether either treatment can mimic the effects of ethylene for experiments that are sensitive to ethylene concentration, response window, and treatment durations. Ethylene can be chemically made by ethanol dehydration; however, further purification from the dehydration products is needed. We previously reported that the ethylene gas can be easily prepared by decomposing ethephon in a buffered condition and the resulting ethylene can be used directly. Ethylene responses can be estimated by the measurement of the hypocotyl length of etiolated seedlings, or by ERF1 (Ethylene Response Factor1) expression. Although ACC of low concentrations is insufficient to induce ERF1 expression, ACC of high concentrations can replace ethylene for experiments where ethylene treatment is not feasible. However, ACC may undergo early consumption. Versatile approaches were developed so that laboratories lacking ethylene and techniques for gas handling can easily perform necessary ethylene treatments.
乙烯是第一个被鉴定出的调节植物生长和发育多方面的气态激素。当乙烯不可用时，ACC（1-氨基环丙烷-1-羧酸）和乙烯利是两种广泛使用的化学物质，可替代乙烯处理。然而，ACC 和乙烯利转化的乙烯量是不可控的，这使得人们怀疑这两种处理方法是否可以模拟对乙烯浓度、响应窗口和处理持续时间敏感的实验的乙烯效应。乙烯可以通过乙醇脱水来化学合成；然而，需要从脱水产物中进一步纯化。我们之前曾报道过，在缓冲条件下分解乙烯利可以很容易地制备乙烯气体，并且可以直接使用所得的乙烯气体。可以通过测量黄化幼苗的下胚轴长度或通过 ERF1（Ethylene Response Factor1，乙烯响应因子 1）表达来估计乙烯响应。尽管低浓度的 ACC 不足以诱导 ERF1 表达，但高浓度的 ACC 可以替代乙烯处理，适用于乙烯处理不可行的实验。然而，ACC 可能会被早期消耗。开发了多种方法，使缺乏乙烯和气体处理技术的实验室能够轻松进行必要的乙烯处理。
