Ye Guan, Tang Yi-Hong, Xia Guang-Xin, Sun Zhao-Lin, Li Zhi-Xiong, Huang Cheng-Gang
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
Biomed Chromatogr. 2010 Nov;24(11):1147-51. doi: 10.1002/bmc.1400.
To profile the anti-Coxsackie virus B3 constituents of Radix Astragali, an HPLC-DAD-MS(n) analytical method, combined with an in vivo test, has been developed to identify the constituents of the active part, which has been demonstrated to have potency to inhibit the proliferation of virus in cardiac muscle, alleviate infraction in heart and elevate the survival rate of the animal. By comparing their retention time and MS data with those obtained from the authentic compounds and the published data, a total of 19 compounds, including 11 isoflavonoids and eight saponins, were identified, among which one pterocarpane glucoside was reported for the first time. The present study provides an approach to rapidly screening bioactive constituents in traditional Chinese medicines.
为了剖析黄芪抗柯萨奇病毒B3的成分，已开发出一种高效液相色谱-二极管阵列检测器-质谱联用（HPLC-DAD-MS(n)）分析方法，并结合体内试验来鉴定活性部位的成分，该活性部位已被证明具有抑制心肌中病毒增殖、减轻心脏梗死和提高动物存活率的能力。通过将它们的保留时间和质谱数据与从标准化合物获得的数据以及已发表的数据进行比较，共鉴定出19种化合物，包括11种异黄酮和8种皂苷，其中一种紫檀烷糖苷为首次报道。本研究提供了一种快速筛选中药中生物活性成分的方法。
