Institute of Technical Biochemistry, University of Stuttgart, Allmandring 31, Stuttgart, Germany.
BMC Biochem. 2010 Feb 1;11:9. doi: 10.1186/1471-2091-11-9.
Thiamine diphosphate (ThDP)-dependent enzymes form a vast and diverse class of proteins, catalyzing a wide variety of enzymatic reactions including the formation or cleavage of carbon-sulfur, carbon-oxygen, carbon-nitrogen, and especially carbon-carbon bonds. Although very diverse in sequence and domain organisation, they share two common protein domains, the pyrophosphate (PP) and the pyrimidine (PYR) domain. For the comprehensive and systematic comparison of protein sequences and structures the Thiamine diphosphate (ThDP)-dependent Enzyme Engineering Database (TEED) was established.
The TEED http://www.teed.uni-stuttgart.de contains 12048 sequence entries which were assigned to 9443 different proteins and 379 structure entries. Proteins were assigned to 8 different superfamilies and 63 homologous protein families. For each family, the TEED offers multisequence alignments, phylogenetic trees, and family-specific HMM profiles. The conserved pyrophosphate (PP) and pyrimidine (PYR) domains have been annotated, which allows the analysis of sequence similarities for a broad variety of proteins. Human ThDP-dependent enzymes are known to be involved in many diseases. 20 different proteins and over 40 single nucleotide polymorphisms (SNPs) of human ThDP-dependent enzymes were identified in the TEED.
The online accessible version of the TEED has been designed to serve as a navigation and analysis tool for the large and diverse family of ThDP-dependent enzymes.
硫胺素二磷酸 (ThDP)-依赖性酶形成了一个庞大而多样的蛋白质类群，催化着各种各样的酶促反应，包括碳-硫、碳-氧、碳-氮，特别是碳-碳键的形成或断裂。尽管它们在序列和结构域组织上非常多样化，但它们都共享两个常见的蛋白质结构域，焦磷酸（PP）和嘧啶（PYR）结构域。为了全面和系统地比较蛋白质序列和结构，建立了硫胺素二磷酸（ThDP）依赖性酶工程数据库（TEED）。
TEED http://www.teed.uni-stuttgart.de 包含 12048 个序列条目，这些序列条目被分配到 9443 个不同的蛋白质和 379 个结构条目。这些蛋白质被分配到 8 个不同的超家族和 63 个同源蛋白家族。对于每个家族，TEED 提供多序列比对、系统发育树和家族特异性 HMM 轮廓。保守的焦磷酸（PP）和嘧啶（PYR）结构域已被注释，这允许对广泛的蛋白质进行序列相似性分析。已知人类 ThDP-依赖性酶与许多疾病有关。在 TEED 中鉴定出 20 种不同的蛋白质和超过 40 种人类 ThDP-依赖性酶的单核苷酸多态性（SNP）。
在线访问版本的 TEED 旨在作为 ThDP-依赖性酶这个庞大而多样的家族的导航和分析工具。
