He Songqing, Atkinson Carl, Qiao Fei, Chen Xiaoping, Tomlinson Stephen
Department of Microbiology and Immunology, Children's Research Institute, Medical University of South Carolina, Charleston, South Carolina, USA.
J Am Assoc Lab Anim Sci. 2010 Jan;49(1):45-51.
Orthotopic liver transplantation in mice and rats is used to study a wide range of scientific questions. Inhalant anesthesia is widely used in liver transplantation models in rodents, but drawbacks of inhalant anesthetics include issues of cost, safety, and ease of use. The goal here was to find an effective injection anesthesia protocol that would not directly influence metabolic or functional parameters after liver transplantation. We compared intraperitoneal injection of a ketamine-xylazine-acepromazine cocktail (KXA) with isoflurane during 50% liver transplantation in mice and rats. Anesthesia with KXA had rapid induction (5 +/- 3 min) and a long duration of surgical anesthesia (70 +/- 10 min). The 2 methods of anesthesia produced no significant differences in liver injury (histology, serum alanine aminotransferase and total bilirubin concentrations), inflammation (IL6, TNFalpha, myeloperoxidase activity), regeneration (incorporation of 5-bromo-2'-deoxyuridine, mitotic index, restitution of liver weight), or 7-d survival. In conclusion, a KXA regimen is a safe and effective injectable anesthetic for rodent liver transplantation and is a suitable substitute for currently used inhalant anesthesia. Injectable anesthetics offer advantages in terms of cost, personal safety, and ease of use and will be particularly beneficial to microsurgeons during their training period in liver transplantation.
小鼠和大鼠的原位肝移植被用于研究广泛的科学问题。吸入麻醉在啮齿动物肝移植模型中被广泛使用，但吸入麻醉剂的缺点包括成本、安全性和易用性等问题。这里的目标是找到一种有效的注射麻醉方案，该方案不会在肝移植后直接影响代谢或功能参数。我们在小鼠和大鼠50%肝移植过程中，将氯胺酮-赛拉嗪-乙酰丙嗪合剂（KXA）腹腔注射与异氟烷进行了比较。KXA麻醉诱导迅速（5±3分钟），手术麻醉持续时间长（70±10分钟）。两种麻醉方法在肝损伤（组织学、血清丙氨酸转氨酶和总胆红素浓度）、炎症（IL6、TNFα、髓过氧化物酶活性）、再生（5-溴-2'-脱氧尿苷掺入、有丝分裂指数、肝重量恢复）或7天生存率方面均无显著差异。总之，KXA方案是一种用于啮齿动物肝移植的安全有效的注射麻醉剂，是目前使用的吸入麻醉的合适替代品。注射麻醉剂在成本、个人安全和易用性方面具有优势，对肝移植培训期的显微外科医生将特别有益。
