Department of Histology and Cell Biology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Acta Histochem Cytochem. 2009 Dec 29;42(6):181-90. doi: 10.1267/ahc.09030. Epub 2009 Dec 22.
Although human papillomavirus (HPV) 16 is the cause of cervical cancer in most countries including Japan, the involvement of cervical cancer with HPV types in Mongolian and Myanmar populations is largely unknown. We examined the expression of HPV in formalin-fixed and paraffin-embedded cervical tissues from 40 Japanese, 32 Mongolian, and 30 Myanmar cervical cancer patients. We performed immunohistochemistry using anti-HPV16 and anti-HPV 1, 6, 11, 16, 18 and 31 cocktail and then correlated it with the expression of Ki-67 and p63. HPV 16 was detected in 72%, 65% and 50% of Japanese, Mongolian and Myanmar cervical cancer patients, respectively, whereas 5 (13%) of the 40 patients, 8 (25%) of the 32 patients and 7 (23%) of the 30 patients in HPV 16-negative cancers were positive for other HPV types included in the cocktail, respectively. Ki-67 labeling index (LI) as well as p63 LI was significantly higher in HPV 16-positive patients than in HPV 16-negative ones in the Japanese and Mongolian samples. p63 expression was significantly associated with stage III and IV in Japan and Mongolia. These findings suggest that HPV 16 may be associated with cell proliferative activity and tumor progression, possibly depending upon the expression of p63 in the cervical cancer. In addition, immunohistochemical detection for distinguishing the type of HPV may also be useful for cervical cancer in the clinical setting.
虽然人乳头瘤病毒（HPV）16 是包括日本在内的大多数国家宫颈癌的病因，但蒙古和缅甸人群中宫颈癌与 HPV 类型的关系在很大程度上尚不清楚。我们检测了 40 例日本、32 例蒙古和 30 例缅甸宫颈癌患者的福尔马林固定石蜡包埋宫颈组织中 HPV 的表达情况。我们使用抗 HPV16 和抗 HPV 1、6、11、16、18 和 31 鸡尾酒进行免疫组织化学染色，然后将其与 Ki-67 和 p63 的表达相关联。HPV16 在日本、蒙古和缅甸宫颈癌患者中的检出率分别为 72%、65%和 50%，而在 HPV16 阴性癌症中，5（13%）例、8（25%）例和 7（23%）例患者的其他 HPV 类型呈阳性，这些类型包含在鸡尾酒检测中。在日本和蒙古的样本中，HPV16 阳性患者的 Ki-67 标记指数（LI）和 p63 LI 均明显高于 HPV16 阴性患者。p63 的表达与日本和蒙古的 III 期和 IV 期显著相关。这些发现表明，HPV16 可能与细胞增殖活性和肿瘤进展有关，这可能取决于 p63 在宫颈癌中的表达。此外，区分 HPV 类型的免疫组织化学检测在临床环境中可能对宫颈癌也有一定的作用。
