Department of Pharmacology and Laboratory of Biotechnology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.
Zoology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt.
Molecules. 2023 Jul 20;28(14):5543. doi: 10.3390/molecules28145543.
Colon cancer is one of the most common types of cancer worldwide, and its incidence is increasing. Despite advances in medical science, the treatment of colon cancer still poses a significant challenge. This study aimed to investigate the potential protective effects of Adiantum pedatum (AP) extract and/or piceatannol on colon cancer induced via phenylhydrazine (PHZ) in terms of the antioxidant and apoptotic pathways and histopathologic changes in the colons of male albino rats. The rats were randomly divided into eight groups: control, AP extract, piceatannol (P), PHZ, PHZ and AP treatments, PHZ and P treatments, PHZ and both AP and P, and PHZ and prophylaxis with both AP and P. The results demonstrated that PHZ induced oxidative damage, apoptosis, and histopathological changes compared to the control group. However, the administration of AP or P or AP + P as therapy or prophylaxis significantly ameliorated these changes and upregulated the colonic mir-145 and mRNA expression of P53 and PDCD-4 while downregulating the colonic mRNA expression of PI3K, AKT, c-Myc, CK-20, SOX-2, OCT-4, and NanoG compared to the PHZ group. These findings suggest that the candidate drugs may exert their anti-cancer effects through multiple mechanisms, including antioxidant and apoptotic activities.
结肠癌是全球最常见的癌症类型之一，其发病率正在上升。尽管医学科学取得了进步，但结肠癌的治疗仍然是一个重大挑战。本研究旨在探讨贯众提取物（AP）和/或白皮杉醇（P）通过苯肼（PHZ）诱导的雄性白化大鼠结肠中的抗氧化和凋亡途径以及组织病理学变化，对结肠癌的潜在保护作用。大鼠随机分为八组：对照组、AP 提取物组、P 组、PHZ 组、PHZ 和 AP 治疗组、PHZ 和 P 治疗组、PHZ 和 AP 和 P 联合治疗组以及 PHZ 和 AP 和 P 联合预防组。结果表明，与对照组相比，PHZ 诱导了氧化损伤、细胞凋亡和组织病理学变化。然而，与 PHZ 组相比，AP 或 P 或 AP+P 作为治疗或预防药物的给药显著改善了这些变化，并上调了 colonic mir-145 和 P53 及 PDCD-4 的 mRNA 表达，同时下调了 colonic mRNA 表达PI3K、AKT、c-Myc、CK-20、SOX-2、OCT-4 和 NanoG。这些发现表明，候选药物可能通过多种机制发挥其抗癌作用，包括抗氧化和凋亡活性。
