Kadhem Mallakh M, Mohammed Mahmood M, Hasan Mohammed Ali S
Department of Medical Laboratory Techniques, Ashur University College, Baghdad, Iraq.
Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
Arch Razi Inst. 2022 Feb 28;77(1):383-390. doi: 10.22092/ARI.2021.356608.1879. eCollection 2022 Feb.
Cancer of the colon (colorectal cancer, or CRC) is the third most frequent malignancy in the world and the fourth leading cause of cancer-related death. Recent research has focused on the link between high-risk human papillomavirus (HPV) infections and the onset/development of several different types of cancer in humans. As a result, scientists are now paying more attention to HPV and CRC. In a variety of malignant tumors, P63 is overexpressed. This includes non-Hodgkin lymphoma and breast carcinoma, as well as lung, bladder, and prostate cancers. However, in accordance with the existence of many P63 isoforms in malignant tumors, the actions of P63 in these malignancies remain a source of debate. P63 immunohistochemistry expression in CRC tissues is being investigated as a possible etiological link between high-risk HPV types and CRC. This retrospective study intended to investigate if there was an etiological link between high-risk HPV types and CRC. It has utilized 92 chosen formalin-fixed and paraffin-embedded tissue block samples. The collected samples were divided into 62 blocks of colorectal adenocarcinoma mass tissues and 30 non-malignant colorectal tissues used as a control group. Chromogenic in situ hybridization (CISH) was employed to discover HPV DNA16/18 in colorectal tissues. The overall proportion of positive HPV16/18 DNA- CISH detection in the mass CRC group was 44.4%, whereas HPV16/18 DNA was obtained at 80.0% in the non-malignant control group. The overall proportion of positive P63-ISH detection in the CRC group was also 70.4%, whereas P63 was 73.3% in the non-malignant control group. The link between HPV infection and P63 expression in CRC might point to the importance of these molecules in the progression of CRC.
结肠癌（结直肠癌，或CRC）是全球第三常见的恶性肿瘤，也是癌症相关死亡的第四大主要原因。最近的研究聚焦于高危型人乳头瘤病毒（HPV）感染与人类几种不同类型癌症的发生/发展之间的联系。因此，科学家们现在更加关注HPV与CRC。在多种恶性肿瘤中，P63过度表达。这包括非霍奇金淋巴瘤、乳腺癌，以及肺癌、膀胱癌和前列腺癌。然而，鉴于恶性肿瘤中存在多种P63亚型，P63在这些恶性肿瘤中的作用仍是一个有争议的问题。正在研究CRC组织中P63免疫组化表达，以探寻高危型HPV与CRC之间可能存在的病因联系。这项回顾性研究旨在调查高危型HPV与CRC之间是否存在病因联系。它使用了92个选定的福尔马林固定石蜡包埋组织块样本。收集的样本分为62个结肠腺癌肿块组织块和30个用作对照组的非恶性结肠组织块。采用显色原位杂交（CISH）法检测结肠组织中的HPV DNA16/18。CRC肿块组中HPV16/18 DNA - CISH检测阳性的总体比例为44.4%，而在非恶性对照组中HPV16/18 DNA的检出率为80.0%。CRC组中P63 - ISH检测阳性的总体比例也为70.4%，而在非恶性对照组中P63为73.3%。CRC中HPV感染与P63表达之间的联系可能表明这些分子在CRC进展中的重要性。
