Department of Science Education, Jeju National University, Jeju 690-756, Republic of Korea.
J Microbiol. 2009 Dec;47(6):710-5. doi: 10.1007/s12275-009-0111-0. Epub 2010 Feb 4.
A novel, Gram-positive bacterium, designated DLS-06(T), was isolated from scoria (volcanic ash) under rock on the peak of small mountain (300 m above the sea level; known as Darangshi Oreum) in Jeju, Republic of Korea. The cells of the isolate were aerobic, oxidase-negative, catalase-positive, endospore-forming, non-motile rods. The organism grew at 25 approximately 30 degrees C and initial pH 6.1 approximately 9.1. A neighbour-joining tree based on 16S rRNA gene sequences showed that the organism was related to members of the family "Sporolactobacillaceae" and related taxa. The phylogenetic neighbours were Pullulanibacillus naganoensis (95.2% 16S rRNA gene sequence similarity), Tuberibacillus calidus (95.0%) and Sporolactobacillus (91.8 approximately 94.2%). Levels of 16S rRNA gene sequence similarity of the isolate to representatives of other genera were in the range of 87.2 approximately 93.7%. The organism contained meso-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The predominant menaquinone was MK-7. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, an unknown ninhydrin-positive phospholipid, three unknown phospholipids and an unknown lipid. The major fatty acids were anteiso-C(15:0) and anteiso-C(17:0). The G+C content of the DNA was 50.8 mol%. On the basis of the phenotypic and phylogenetic data presented in this study, this organism represents a novel genus and species in the order Bacillales, for which the name Scopulibacillus darangshiensis gen. nov., sp. nov. is proposed. The type strain is DLS-06(T) (=DSM 19377(T) =KCTC 13161(T)).
一种新型革兰氏阳性细菌，被命名为 DLS-06(T)，是从韩国济州岛的小山顶（海拔 300 米）的岩石下的火山灰（浮石）中分离出来的。该分离株的细胞为需氧、氧化酶阴性、过氧化氢酶阳性、芽孢形成、非运动杆状。该生物在 25 摄氏度左右至 30 摄氏度和初始 pH 值 6.1 左右至 9.1 下生长。基于 16S rRNA 基因序列的邻接法树表明，该生物与“芽孢杆菌科”和相关分类群的成员有关。其系统发育上的近邻是 Pullulanibacillus naganoensis（95.2%的 16S rRNA 基因序列相似性）、Tuberibacillus calidus（95.0%）和 Sporolactobacillus（91.8 至 94.2%）。该分离株与其他属代表的 16S rRNA 基因序列相似性水平在 87.2 至 93.7%之间。该生物的细胞壁肽聚糖中含有 meso-二氨基庚二酸作为诊断性二氨基庚二酸。主要的menaquinone 是 MK-7。极性脂谱含有双磷脂酰甘油、磷脂酰甘油、一种未知的茚三酮阳性磷脂、三种未知的磷脂和一种未知的脂类。主要脂肪酸为 anteiso-C(15:0)和 anteiso-C(17:0)。DNA 的 G+C 含量为 50.8 mol%。基于本研究中呈现的表型和系统发育数据，该生物代表了一个新的属和目 Bacillales 的种，命名为 Scopulibacillus darangshiensis 属。新种，模式菌株为 DLS-06(T)（=DSM 19377(T) =KCTC 13161(T)）。
